Pérez-Santonja J J, Linna T U, Tervo K M, Sakla H F, Alió y Sanz J L, Tervo T M
Department of Ophthalmology, University of Alicante, Spain.
J Refract Surg. 1998 Nov-Dec;14(6):602-9. doi: 10.3928/1081-597X-19981101-06.
The aim of this study was to characterize the cell biology of wound healing in rabbit corneas subjected to laser in situ keratomileusis (LASIK).
Rabbit corneas underwent LASIK with various multizone photoablations or only a lamellar keratotomy followed by repositioning of the flap. We looked for indications for an active wound healing process. Immunohistochemistry for the extradomain A cellular fibronectin (EDA-cFn) or tenascin (Tn) and routine histology were examined.
Four days after LASIK or lamellar keratotomy followed by repositioning of the flap, epithelial plugs and prominent keratocytes as well as Tn and EDA-cFn immunoreactions-indicative of a wound-healing process-appeared in the wound margins. Epithelial plugs were less conspicous, and prominent, presumably activated, keratocytes were no longer identified at the wound margin at 2.5 and 5 months after wounding. However, EDA-cFn and Tn immunoreactivities could still be observed. Only the stromal cells located in the periphery of the flap and in relatively close contact with the epithelium were surrounded by scar tissue expressing immunoreactivity for EDA-cFn or Tn. The central corneal stroma was devoid of scar tissue.
Results indicate that the wound healing reaction after LASIK takes place only at the periphery of the microkeratome wound, leaving the central optical zone clear.
本研究旨在描述接受准分子原位角膜磨镶术（LASIK）的兔角膜伤口愈合的细胞生物学特征。
兔角膜接受不同的多区光凝消融或仅行板层角膜切开术，然后将角膜瓣复位。我们寻找活跃伤口愈合过程的迹象。检测细胞外区域A细胞纤连蛋白（EDA-cFn）或肌腱蛋白（Tn）的免疫组织化学及常规组织学。
LASIK或板层角膜切开术并复位角膜瓣4天后，伤口边缘出现上皮栓、显著的角膜细胞以及Tn和EDA-cFn免疫反应，提示伤口愈合过程。在受伤后2.5个月和5个月时，上皮栓不那么明显，伤口边缘不再能识别出显著的、可能被激活的角膜细胞。然而，仍可观察到EDA-cFn和Tn免疫反应性。仅位于角膜瓣周边且与上皮相对紧密接触的基质细胞被表达EDA-cFn或Tn免疫反应性的瘢痕组织包围。角膜中央基质无瘢痕组织。
结果表明，LASIK术后的伤口愈合反应仅发生在微型角膜刀伤口的周边，使中央光学区保持清晰。
