Latvala T, Tervo K, Mustonen R, Tervo T
University of Helsinki, Department of Ophthalmology, Finland.
Br J Ophthalmol. 1995 Jan;79(1):65-9. doi: 10.1136/bjo.79.1.65.
An indirect immunohistochemical technique was used to monitor the expression of cellular fibronectin (cFN) and tenascin (TN) in the rabbit cornea after photorefractive keratectomy (PRK) in a 1 year follow up study. Rabbits received a 5.0 D myopic PRK, and were killed 3 days, 1, 3, 6, or 12 months after the operation. In most corneas, secondary epithelial defects appeared after the primary healing (mean 6.3 (SD 1.2) days). Corneal haze appeared a few weeks after PRK and was observed throughout the follow up. Three days after wounding an immunoreaction for cFN was observed as a bright narrow subepithelial line, but no immunoreaction for TN could be seen in the anterior third of the corneal stroma. However, at 1-6 months a similar location of immunoreactions for both cFN and TN was observed. Both were found in the anterior stroma at depths of 30-50 microns. At 12 months, only a trace of cFN immunoreaction but no TN immunoreaction could be discerned. Our results suggest that subepithelial scar tissue contains both cFN and TN up to 12 months.
在一项为期1年的随访研究中，采用间接免疫组织化学技术监测了兔屈光性角膜切削术（PRK）后角膜中细胞纤连蛋白（cFN）和腱生蛋白（TN）的表达。兔接受了5.0 D的近视PRK手术，并在术后3天、1、3、6或12个月处死。在大多数角膜中，原发性愈合后出现继发性上皮缺损（平均6.3（标准差1.2）天）。PRK术后几周出现角膜混浊，并在整个随访过程中均有观察到。受伤后3天，观察到cFN的免疫反应呈明亮的窄带状位于上皮下，但在角膜基质前三分之一处未见TN的免疫反应。然而，在1至6个月时，观察到cFN和TN的免疫反应位于相似位置。二者均在前基质中30至50微米深度处被发现。在12个月时，仅能辨别出微量的cFN免疫反应，但未见TN免疫反应。我们的结果表明，上皮下瘢痕组织在长达12个月的时间内同时含有cFN和TN。
