Vishnevskaia E B
Probl Tuberk. 1998(5):40-2.
While testing samples by the polymerase chain reaction (PCR), a procedure for DNA isolation is advisable adapted for different types of materials; thorough DNA purification is of the greatest importance in testing blood-containing samples, deep sample lysis is of particular importance for osseous tissue samples. A differential approach to isolating DNA from clinical samples allows the efficiency of PCR to be enhanced.
在通过聚合酶链反应(PCR)检测样本时,建议针对不同类型的材料采用适合的DNA分离程序;在检测含血样本时,彻底的DNA纯化至关重要,而对于骨组织样本,深度样本裂解尤为重要。采用差异化方法从临床样本中分离DNA可提高PCR的效率。