Li J, Yang Q, Hu S
Institute of Psychiatry, Xiangya Hospital, Hunan Medical University, Shangsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(1):19-21.
We observed the changes of excitatory amino acids (EAA) in extracellular fluid (ECLF) in rat hippocampus in case of cerebral ischemia after giving Wuto Granule compring the test groups with the control groups by microdialysis method. Results showed that there was no effect on EAA in extrcellular fluid by the drug before cerebral ischemia, but there existed staistical singnifcant changes of EAA in extracellular fluid one hour after cerebral ischemia comparing the test groups Glu and Asp with the control groups (P < 0.01). We found that there is a decrease of release of release of EAA in ECLF in case of ischemia by Wuto Granule. It could be deduced that Wuto Granule can penetrate the brain-blood barrier and this can protect the brain incse of ischemia. The application of intracerebral micrdialysis has provided a new method for the research work of pharmacology.
采用微透析法，将试验组与对照组比较，观察了五藤颗粒对大鼠脑缺血时海马细胞外液中兴奋性氨基酸（EAA）的变化。结果显示，给药前药物对细胞外液中EAA无影响，但脑缺血1小时后，试验组的谷氨酸（Glu）和天冬氨酸（Asp）与对照组比较，细胞外液中EAA存在统计学显著变化（P<0.01）。我们发现，五藤颗粒可使缺血时细胞外液中EAA释放减少。可以推断，五藤颗粒能透过血脑屏障，对脑缺血具有保护作用。脑内微透析的应用为药理学研究工作提供了一种新方法。
