Feng D, Cheng R, Yan Y
Department of Pathology, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(4):310-2.
Expression of p21 and c-myc protein in hepatocellular carcinomas and their surrounding liver tissue was detected on serial sections by immunohistochemical method. The results showed that the positive rates of p21 expression were 53.3% (16/30) and 96.7% (29/30) in hepatocellular carcinomas and pericarcinomatous liver tissue, and 40% (12/30) and 86.7% (26/30) and 86.7% (26/30) for c-myc protein expression respectively. Their incidences in pericarcinomatous liver tissue were higher than that in cancer tissue (P < 0.01). The patterns of p21 and c-myc protein in cells were cytoplasm, membrane and/or nuclear types. Their expression was more intensive in pericarcinomatous hepatocytes, especially in liver cirrhosis nodes. The results indicate that abnormal activiation and expression of oncogene ras and c-myc may be related to hepatocellular carcinogenesis.
采用免疫组化方法在连续切片上检测肝细胞癌及其周围肝组织中p21和c-myc蛋白的表达。结果显示，p21表达的阳性率在肝细胞癌和癌旁肝组织中分别为53.3%（16/30）和96.7%（29/30），c-myc蛋白表达的阳性率分别为40%（12/30）、86.7%（26/30）和86.7%（26/30）。其在癌旁肝组织中的发生率高于癌组织（P<0.01）。p21和c-myc蛋白在细胞中的表达模式为胞质型、膜型和/或核型。它们在癌旁肝细胞中的表达更强，尤其是在肝硬化结节中。结果表明，癌基因ras和c-myc的异常激活和表达可能与肝细胞癌的发生有关。
