Ouyang F, Peng H, Wu J, Pu P
Clinical Laboratory, Third Affiliated Hospital, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(4):357-9.
By using the indirect immunofluorescence assay and the tissue cells infected with coxackie virus Group B as antigen, the authors detected the antibodies type IgM to coxsackie virus Group B (CVB-IgM) of the sera of 105 children with viral myocarditis (VMC), 59 children with other diseases (COD) and 67 healthy children (HC). The results showed that in VMC, the genometric mean titre (GMT) of CVB-IgM in female (1:16.58) was higher than that in male (1:9.28, P < 0.05); not only in female, but also in male the GMT of VMC was higher than that of HC; also the GMT in female of VMC was higher than the GMT in female of COD, but no considerable difference existed between male of VMC and COD. Without sex distinction the GMT of HC was 0.289, its standard variance was 0.9335, the upper limit titre of normal range was 1:8.6. The authors took 1:10 as the positive criterion, the sensitivity of CVB-IgM in diagnosing VMC was 79.05%, the specialities of differentiating VMC from COD and HC were 42.3% and 91.04%, the consistancy rates of diagnosing VMC from COD and HC were 65.85% and 83.72% respectively.
作者以柯萨奇B组病毒感染的组织细胞为抗原，采用间接免疫荧光法，检测了105例病毒性心肌炎（VMC）患儿、59例其他疾病（COD）患儿及67例健康儿童（HC）血清中柯萨奇B组病毒IgM抗体（CVB-IgM）。结果显示，VMC组中，女性CVB-IgM几何平均滴度（GMT）（1:16.58）高于男性（1:9.28，P<0.05）；VMC组无论男性还是女性的GMT均高于HC组；VMC组女性的GMT高于COD组女性，但VMC组男性与COD组男性之间无显著差异。不区分性别时，HC组的GMT为0.289，标准差为0.9335，正常范围上限滴度为1:8.6。作者以1:10为阳性标准，CVB-IgM诊断VMC的敏感性为79.05%，鉴别VMC与COD及HC的特异性分别为42.3%和91.04%，诊断VMC与COD及HC的符合率分别为65.85%和83.72%。
