Kordová N, Wilt J C
Can J Microbiol. 1976 Aug;22(8):1169-80. doi: 10.1139/m76-170.
Phagocytic and chamydiacidal properties of nonstimulated and stimulated mouse mononuclear cells for two Chlamydia psittaci 6BC strains were investigated. It was determined that macrophages kept in monolayer culture (i.e. stimulated phagocytes) developed much more efficient chlamydiacidal ability than did cells kept in suspension directly after harvest (i.e. nonstimulated phagocytes). A thousandfold decrease of chlamydial infectivity was observed 60 min after induction of phagocytosis in stimulated macrophages, irrespective of the strain offered. In contrast, the infectivity of both chlamydial strains remained essentially unchanged up to 1 h after phagcoytosis by nonstimulated phagocytes. Electron-microscopic examination indicated that chlamydiae were disintegrated within phagolysosomes of stimulated phagocytes within minutes after phagocytosis, although ultrastructurally altered chlamydiae not enclosed in vacuoles were also observed in the cytoplasm of several mononuclear phagocytes at different times after phagocytosis.
研究了未刺激和刺激后的小鼠单核细胞对两种鹦鹉热衣原体6BC菌株的吞噬和杀衣原体特性。结果表明，单层培养的巨噬细胞（即刺激后的吞噬细胞）比收获后直接悬浮保存的细胞（即未刺激的吞噬细胞）具有更高的杀衣原体能力。在刺激后的巨噬细胞中，吞噬作用诱导60分钟后，无论提供何种菌株，衣原体感染性均下降千倍。相比之下，未刺激的吞噬细胞吞噬后1小时内，两种衣原体菌株的感染性基本保持不变。电子显微镜检查表明，吞噬后几分钟内，衣原体在刺激后吞噬细胞的吞噬溶酶体内解体，尽管在吞噬后不同时间，几个单核吞噬细胞的细胞质中也观察到未被液泡包裹的超微结构改变的衣原体。
