Michel E, Mengaud J, Galsworthy S, Cossart P
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.
FEMS Microbiol Lett. 1998 Dec 15;169(2):341-7. doi: 10.1111/j.1574-6968.1998.tb13338.x.
Through the analysis of a non-motile mutant of Listeria monocytogenes, we identified and characterized a locus containing the cheR, motA and motB genes. These three genes are homologous to the cheR, and motA/B genes of Bacillus subtilis which in this organism are 954 kb apart. The gene organization in Listeria is also not similar either to that of Escherichia coli in which cheR and motAB are 5.9 kb apart. CheR and motA/B, as previously reported for flaA, the flagellin gene, are thermoregulated with a higher expression at 25 degrees C and low expression at 37 degrees C. In a delta prfA strain, motA expression was derepressed at 37 degrees C, suggesting that PrfA, the transcriptional activator of virulence genes, downregulates motility genes in Listeria at 37 degrees C.
通过对单核细胞增生李斯特菌的一个非运动性突变体进行分析，我们鉴定并表征了一个包含cheR、motA和motB基因的位点。这三个基因与枯草芽孢杆菌的cheR以及motA/B基因同源，在该生物体中它们相距954 kb。李斯特菌中的基因组织也与大肠杆菌不同，在大肠杆菌中cheR和motAB相距5.9 kb。如先前关于鞭毛蛋白基因flaA的报道，CheR和motA/B受温度调节，在25℃时表达较高，在37℃时表达较低。在一个缺失prfA的菌株中，motA在37℃时的表达解除抑制，这表明毒力基因的转录激活因子PrfA在37℃时下调李斯特菌中的运动基因。
