Nádházi Z, Takáts A, Offenmüller K, Bertók L
II. Belgyógyászati Klinika, Semmelweis Orvostudományi Egyetem, Budapest.
Orv Hetil. 1998 Nov 29;139(48):2889-91.
The plasma level of endotoxin was determined in 116 healthy blood donors in this laboratory. After a routine physical and laboratory investigations the endotoxin level was determined with Limulus amebocyte lysate assay (LAL test) by the chromogenic kinetic method of Bio-Whittaker Co. (USA). Its sensitivity was 0.005-50 EU/ml. The plasma level of endotoxin in most of healthy donors was less than 1 EU/ml (in the range of 0.01-1.0 EU/ml), but always measurable. The average +/- S. D. was 0.128 +/- 0.215 EU/ml. Because of the high standard deviation and high range of values, the data were distributed to two groups with the means of 0.05 +/- 0.022 EU/ml and 0.294 +/- 0.186 EU/ml. The difference between the groups was significant found (p < 0.001). In conclusion, endotoxin can be measured in plasma of healthy individuals.
本实验室对116名健康献血者的血浆内毒素水平进行了测定。在进行常规体格检查和实验室检查后，采用美国Bio-Whittaker公司的显色动力学方法，通过鲎试剂法（LAL试验）测定内毒素水平。其灵敏度为0.005 - 50 EU/ml。大多数健康献血者的血浆内毒素水平低于1 EU/ml（在0.01 - 1.0 EU/ml范围内），但始终可测。平均±标准差为0.128±0.215 EU/ml。由于标准差高且数值范围大，数据被分为两组，均值分别为0.05±0.022 EU/ml和0.294±0.186 EU/ml。两组之间的差异具有显著性（p < 0.001）。总之，健康个体的血浆中可检测到内毒素。
