Martínez Vidal J L, Arrebola F J, Fernández-Gutiérrez A, Rams M A
Department of Analytical Chemistry, University of Almería, Spain.
J Chromatogr B Biomed Sci Appl. 1998 Nov 20;719(1-2):71-8. doi: 10.1016/s0378-4347(98)00411-3.
A method was developed for determining the occupational exposure to endosulfan and its main metabolites (endosulfanether, -lactone and -sulfate) in human urine using dieldrin as an internal standard. Solid phase extraction (SPE) and gas chromatography-tandem mass spectrometry (GC-MS-MS) have been used due to their high sensitivity and selectivity in avoiding most matrix interferences. The recovery efficiencies of the tested compounds yielded more than 89.2% at the fortification level of 10 ng ml(-1) in urine and their relative standard deviations were between 9.1 and 12.8%. The detection limit of each compound ranged between 6 and 18 pg ml(-1). Urine samples from nine pest control operators were analysed and total endosulfan concentrations between 94 and 2038 pg ml(-1) were found.
开发了一种以狄氏剂为内标物测定人尿中硫丹及其主要代谢物（硫丹醚、硫丹内酯和硫丹硫酸盐）职业暴露量的方法。采用固相萃取（SPE）和气相色谱 - 串联质谱（GC-MS-MS），因其具有高灵敏度和选择性，可避免大多数基质干扰。在尿样中添加浓度为10 ng ml(-1)时，所测化合物的回收率超过89.2%，其相对标准偏差在9.1%至12.8%之间。每种化合物的检测限在6至18 pg ml(-1)之间。分析了9名害虫防治操作人员的尿样，发现硫丹总浓度在94至2038 pg ml(-1)之间。
