验证的高效液相色谱法测定人血浆中丙嗪。在药代动力学研究中的应用。
Validated high-performance liquid chromatographic assay for the determination of promazine in human plasma. Application to pharmacokinetic studies.
作者信息
Larsimont V, Meins J, Fieger-Büschges H, Blume H
机构信息
Zentrallaboratorium Deutscher Apotheker, Eschborn, Germany.
出版信息
J Chromatogr B Biomed Sci Appl. 1998 Nov 20;719(1-2):222-6. doi: 10.1016/s0378-4347(98)00262-x.
A high-performance liquid chromatographic method for the determination of promazine in human plasma is described. The assay involves a single-step liquid-liquid extraction using pentane-2-propanol (98:2, v/v). The analyte of interest and the internal standard chlorpromazine were separated on a Spherisorb CN column using a mobile phase of acetonitrile-50 mM ammonium acetate (9:1, v/v). Electrochemical detection was achieved using an applied potential of +750 mV. The assay was validated according to international requirements prior to application to a pharmacokinetic study and was found to be specific, accurate and precise with a linear range of 0.25-25 ng ml(-1).
本文描述了一种测定人血浆中丙嗪的高效液相色谱法。该测定方法包括使用戊烷 - 2 - 丙醇（98:2，v/v）进行单步液 - 液萃取。目标分析物和内标氯丙嗪在Spherisorb CN柱上分离，流动相为乙腈 - 50 mM醋酸铵（9:1，v/v）。通过施加 +750 mV的电位实现电化学检测。该测定方法在应用于药代动力学研究之前根据国际要求进行了验证，结果表明其具有特异性、准确性和精密度，线性范围为0.25 - 25 ng ml⁻¹。
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