Le Guellec C, Gaudet M L, Breteau M
Laboratory of Pharmacology and Toxicology, CHU Bretonneau, Tours, France.
J Chromatogr B Biomed Sci Appl. 1998 Nov 20;719(1-2):227-33. doi: 10.1016/s0378-4347(98)00391-0.
We report a high-performance liquid chromatography method for clonazepam determination in plasma. The use of a synthetic silica-based stationary phase markedly improved clonazepam resolution compared to standard reversed-phase columns. A liquid-liquid extraction was used, associated with reversed-phase chromatography, gradient elution and ultraviolet detection. Accuracy and precision were satisfactory at therapeutic concentrations. Selectivity was studied for benzodiazepines or other antiepileptic drugs, with particular attention to newly marketed drugs i.e., gabapentine and vigabatrin. No interfering substance was evidenced. Under the conditions described, it was possible to quantify clonazepam at nanogram level even when carbamazepine was present at therapeutic concentrations.
我们报告了一种用于测定血浆中氯硝西泮的高效液相色谱法。与标准反相柱相比，使用基于合成硅胶的固定相显著提高了氯硝西泮的分离度。采用液-液萃取，并结合反相色谱、梯度洗脱和紫外检测。在治疗浓度下，准确度和精密度令人满意。研究了该方法对苯二氮䓬类药物或其他抗癫痫药物的选择性，特别关注新上市的药物，即加巴喷丁和氨己烯酸。未发现干扰物质。在所描述的条件下，即使卡马西平处于治疗浓度，也能够对纳克水平的氯硝西泮进行定量。
