Peck S C, Kende H
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48823-1312, USA.
Plant Mol Biol. 1998 Dec;38(6):977-82. doi: 10.1023/a:1006033030081.
Treatment of 5- to 6-day-old etiolated pea (Pisum sativum L.) seedlings with indole-3-acetic acid (IAA) induced within 15 min an increase in the transcript levels of two genes encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase, Ps-ACS1 and Ps-ACS2. Simultaneous treatment with ethylene inhibited this increase and also caused a decrease in ACC synthase enzyme activity as compared to that of seedlings treated with IAA alone. These results indicate that ethylene inhibits its own biosynthesis by decreasing ACC synthase transcript levels via a negative feedback loop. Wounding of pea stems had no effect on the expression of Ps-ACS1, but led within 10 min to an increase in the mRNA levels of Ps-ACS2. This increase was also inhibited by ethylene. The wound signal was transmitted over a distance of at least 4 cm through the stem with no delay in induction or response intensity. The rapid transmission of the wound response is consistent with the possibility that a hydraulic or electric signal is responsible for the spread of the wound response.
用吲哚 - 3 - 乙酸（IAA）处理5至6日龄的黄化豌豆（Pisum sativum L.）幼苗，15分钟内就会诱导两个编码1 - 氨基环丙烷 - 1 - 羧酸（ACC）合酶的基因Ps - ACS1和Ps - ACS2的转录水平升高。与单独用IAA处理的幼苗相比，同时用乙烯处理会抑制这种升高，并且还会导致ACC合酶活性降低。这些结果表明，乙烯通过负反馈回路降低ACC合酶转录水平来抑制其自身的生物合成。豌豆茎的创伤对Ps - ACS1的表达没有影响，但在10分钟内导致Ps - ACS2的mRNA水平升高。这种升高也受到乙烯的抑制。创伤信号通过茎至少传播4厘米的距离，诱导或反应强度没有延迟。创伤反应的快速传播与液压或电信号负责创伤反应传播的可能性一致。
