Dubois A, Berg D E, Incecik E T, Fiala N, Heman-Ackah L M, Del Valle J, Yang M, Wirth H P, Perez-Perez G I, Blaser M J
Laboratory of Gastrointestinal and Liver Studies, Digestive Diseases Division, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799, USA.
Gastroenterology. 1999 Jan;116(1):90-6. doi: 10.1016/s0016-5085(99)70232-5.
BACKGROUND & AIMS: The specificity of colonization by Helicobacter pylori and complex host-bacterium interactions cannot be readily examined in humans. The aim of this study was to perform such analyses in rhesus monkeys.
Four animals that had been cured of natural H. pylori colonization were challenged with a mixture of 7 strains of human origin, and bacteria recovered during periodic videogastroscopy were DNA fingerprinted.
Three animals carried mixtures of several strains for 4 months, after which strain J166 predominated. In the fourth animal, only strain J238 was isolated from the earliest phase of colonization through 7 months, but strain J166 again became predominant by 10 months after the challenge. Gastritis scores and plasma gastrin and anti-H. pylori immunoglobulin G titers reached levels observed in naturally colonized animals by 4 months after the challenge; however, no plasma immunoglobulin A response was observed up to 10 months.
These results show that (1) natural colonization does not elicit protective immunity against subsequent H. pylori challenge; (2) individuals differ in susceptibility to different H. pylori strains during initial stages of colonization; and (3) certain strains are better suited than others for long-term survival in different hosts. These observations show the complexity of H. pylori-host interactions.
幽门螺杆菌定植的特异性以及复杂的宿主-细菌相互作用难以在人类中轻易进行研究。本研究的目的是在恒河猴中进行此类分析。
对4只已治愈自然幽门螺杆菌定植的动物用7株人类来源的菌株混合物进行攻击，在定期视频胃镜检查期间回收的细菌进行DNA指纹分析。
3只动物携带多种菌株的混合物达4个月，之后J166菌株占主导。在第4只动物中，从定植最早阶段到7个月仅分离出J238菌株，但在攻击后10个月J166菌株再次占主导。攻击后4个月时，胃炎评分、血浆胃泌素和抗幽门螺杆菌免疫球蛋白G滴度达到自然定植动物中观察到的水平；然而，直至10个月均未观察到血浆免疫球蛋白A反应。
这些结果表明：（1）自然定植不会引发针对后续幽门螺杆菌攻击的保护性免疫；（2）个体在定植初始阶段对不同幽门螺杆菌菌株的易感性存在差异；（3）某些菌株比其他菌株更适合在不同宿主中长期存活。这些观察结果显示了幽门螺杆菌-宿主相互作用的复杂性。
