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本文引用的文献

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Transcriptional fidelity and proofreading by RNA polymerase II.RNA聚合酶II的转录保真度与校对功能。
Cell. 1998 May 15;93(4):627-37. doi: 10.1016/s0092-8674(00)81191-5.
2
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution.噬菌体T7 DNA复制复合物的晶体结构，分辨率为2.2埃。
Nature. 1998 Jan 15;391(6664):251-8. doi: 10.1038/34593.
3
Trajectory of DNA in the RNA polymerase II transcription preinitiation complex.RNA聚合酶II转录起始前复合物中DNA的轨迹。
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12268-73. doi: 10.1073/pnas.94.23.12268.
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Two conformations of RNA polymerase II revealed by electron crystallography.通过电子晶体学揭示的RNA聚合酶II的两种构象。
J Mol Biol. 1997 Oct 3;272(4):536-40. doi: 10.1006/jmbi.1997.1273.
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Substrate specificity of the RNase activity of yeast RNA polymerase III.酵母RNA聚合酶III的核糖核酸酶活性的底物特异性
J Biol Chem. 1997 Sep 5;272(36):22832-9. doi: 10.1074/jbc.272.36.22832.
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Basic mechanisms of transcript elongation and its regulation.转录延伸的基本机制及其调控。
Annu Rev Biochem. 1997;66:117-72. doi: 10.1146/annurev.biochem.66.1.117.
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Transcriptional termination signals for RNA polymerase II in fission yeast.裂殖酵母中RNA聚合酶II的转录终止信号
EMBO J. 1997 Jun 16;16(12):3633-43. doi: 10.1093/emboj/16.12.3633.
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Factor requirements for transcription in the Archaeon Sulfolobus shibatae.嗜热栖热硫化叶菌转录所需的因子
EMBO J. 1997 May 15;16(10):2927-36. doi: 10.1093/emboj/16.10.2927.
9
Transcription elongation through DNA arrest sites. A multistep process involving both RNA polymerase II subunit RPB9 and TFIIS.通过DNA停滞位点的转录延伸。这是一个涉及RNA聚合酶II亚基RPB9和TFIIS的多步骤过程。
J Biol Chem. 1997 Jun 6;272(23):14747-54. doi: 10.1074/jbc.272.23.14747.
10
Transcript cleavage in an RNA polymerase I elongation complex. Evidence for a dissociable activity similar to but distinct from TFIIS.RNA聚合酶I延伸复合物中的转录本切割。存在一种与TFIIS相似但不同的可解离活性的证据。
J Biol Chem. 1997 Apr 4;272(14):9055-61. doi: 10.1074/jbc.272.14.9055.

RNA聚合酶III的RNA切割活性由一个必需的类TFIIS亚基介导，对转录终止很重要。

The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination.

作者信息

Chédin S, Riva M, Schultz P, Sentenac A, Carles C

机构信息

Service de Biochimie et de Génétique Moléculaire, CEA/Saclay, F-91191 Gif sur Yvette Cedex, France.

出版信息

Genes Dev. 1998 Dec 15;12(24):3857-71. doi: 10.1101/gad.12.24.3857.

DOI:10.1101/gad.12.24.3857
PMID:9869639
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC317263/
Abstract

Budding yeast RNA polymerase III (Pol III) contains a small, essential subunit, named C11, that is conserved in humans and shows a strong homology to TFIIS. A mutant Pol III, heterocomplemented with Schizosaccharomyces pombe C11, was affected in transcription termination in vivo. A purified form of the enzyme (Pol III Delta), deprived of C11 subunit, initiated properly but ignored pause sites and was defective in termination. Remarkably, Pol III Delta lacked the intrinsic RNA cleavage activity of complete Pol III. In vitro reconstitution experiments demonstrated that Pol III RNA cleavage activity is mediated by C11. Mutagenesis in C11 of two conserved residues, which are critical for the TFIIS-dependent cleavage activity of Pol II, is lethal. Immunoelectron microscopy data suggested that C11 is localized on the mobile thumb-like stalk of the polymerase. We propose that C11 allows the enzyme to switch between an RNA elongation and RNA cleavage mode and that the essential role of the Pol III RNA cleavage activity is to remove the kinetic barriers to the termination process. The integration of TFIIS function into a specific Pol III subunit may stem from the opposite requirements of Pol III and Pol II in terms of transcript length and termination efficiency.

摘要

出芽酵母RNA聚合酶III（Pol III）含有一个名为C11的小的必需亚基，该亚基在人类中保守，并且与TFIIS具有很强的同源性。一种与粟酒裂殖酵母C11异源互补的突变型Pol III在体内转录终止方面受到影响。一种去除了C11亚基的纯化形式的酶（Pol III Delta）能够正常起始，但忽略了暂停位点，并且在终止方面存在缺陷。值得注意的是，Pol III Delta缺乏完整Pol III的内在RNA切割活性。体外重组实验表明，Pol III的RNA切割活性由C11介导。对C11中两个保守残基进行诱变，这两个残基对Pol II的TFIIS依赖性切割活性至关重要，结果是致死的。免疫电子显微镜数据表明，C11定位于聚合酶的可移动拇指状柄上。我们提出，C11允许酶在RNA延伸和RNA切割模式之间切换，并且Pol III RNA切割活性的重要作用是消除终止过程的动力学障碍。将TFIIS功能整合到特定的Pol III亚基中可能源于Pol III和Pol II在转录本长度和终止效率方面的相反要求。