一种新型的依赖RNA聚合酶I的核糖核酸酶活性，可从3'端缩短新生转录本。
A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3' end.
作者信息
Tschochner H
机构信息
Institut für Biochemie I, Universität Heidelberg, Germany.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12914-9. doi: 10.1073/pnas.93.23.12914.
Abstract
A novel RNase activity was identified in a yeast RNA polymerase I (pol I) in vitro transcription system. Transcript cleavage occurred at the 3' end and was dependent on the presence of ternary pol I/DNA/RNA complexes and an additional protein factor not identical to transcription factor IIS (TFIIS). Transcript cleavage was observed both on arrested complexes at the linearized ends of the transcribed DNA and on intrinsic blocks of the DNA template. Shortened transcripts that remained associated within the ternary complexes were capable of resuming RNA chain elongation. Possible functions of the nuclease for transcript elongation or termination are discussed.
摘要
在酵母RNA聚合酶I（pol I）体外转录系统中鉴定出一种新型核糖核酸酶活性。转录本切割发生在3'末端，并且依赖于三元pol I/DNA/RNA复合物以及一种与转录因子IIS（TFIIS）不同的额外蛋白质因子的存在。在转录DNA线性化末端的停滞复合物以及DNA模板的内在障碍处均观察到转录本切割。保留在三元复合物中的缩短转录本能够恢复RNA链延伸。讨论了核酸酶在转录本延伸或终止方面的可能功能。
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本文引用的文献
1
2
3
Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.RNA聚合酶II通过一种序列特异性DNA结合蛋白进行的延伸因子SII依赖性转录。
Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1917-21. doi: 10.1073/pnas.90.5.1917.
4
Transcript cleavage factors from E. coli.来自大肠杆菌的转录物切割因子。
Cell. 1993 Feb 12;72(3):459-66. doi: 10.1016/0092-8674(93)90121-6.
5
Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.与人类RNA聚合酶II相关的3'→5'核酸外切酶活性的鉴定。
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):843-7. doi: 10.1073/pnas.90.3.843.
6
7
Multiple RNA polymerase conformations and GreA: control of the fidelity of transcription.多种RNA聚合酶构象与GreA：转录保真度的控制
Science. 1993 Nov 5;262(5135):867-73. doi: 10.1126/science.8235608.
8
The active site of RNA polymerase II participates in transcript cleavage within arrested ternary complexes.RNA聚合酶II的活性位点参与停滞三元复合物内的转录物切割。
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9
Discontinuous mechanism of transcription elongation.转录延伸的不连续机制。
Science. 1994 Aug 5;265(5173):793-6. doi: 10.1126/science.8047884.
10
A model for transcription termination by RNA polymerase I.RNA聚合酶I介导的转录终止模型。
Cell. 1994 Nov 4;79(3):527-34. doi: 10.1016/0092-8674(94)90261-5.
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