将血管活性肠肽（VIP）反义寡脱氧核苷酸注入下丘脑视交叉上核上方的第三脑室对大鼠颅内注射2-脱氧-D-葡萄糖所致高血糖的影响。

Effect of infusion of vasoactive intestinal peptide (VIP)-antisense oligodeoxynucleotide into the third cerebral ventricle above the hypothalamic suprachiasmatic nucleus on the hyperglycemia caused by intracranial injection of 2-deoxy-D-glucose in rats.

作者信息

Chun S, Niijima A, Shima T, Okada M, Nagai K

机构信息

Division of Protein Metabolism, Institute for Protein Research, Osaka University, Suita, Japan.

出版信息

Neurosci Lett. 1998 Dec 4;257(3):135-8. doi: 10.1016/s0304-3940(98)00831-3.

DOI:10.1016/s0304-3940(98)00831-3

PMID:9870338

Abstract

We examined the effect of the infusion of a vasoactive intestinal peptide (VIP) antisense oligodeoxynucleotide into the third cerebral ventricle above the hypothalamic suprachiasmatic nucleus (SCN) using osmotic minipump for 3 days (0.2 nmol/ml per h) on the hyperglycemic response to intracerebroventricular injection of 2-deoxy-D-glucose (2DG) (80 micromol) in rats. After the infusion of the VIP antisense the inhibition of VIP expression in the SCN was observed in association with suppressions of the hyperglycemia, hyperglucagonemia and relative hypoinsulinemia due to the 2DG injection. Furthermore, additional intracranial injection of VIP (4 nmol) restored these responses to the 2DG injection in rats treated with the VIP antisense. These findings suggest that VIP neurons in the SCN are involved in the regulation of glucose metabolism.

摘要

我们使用渗透微型泵，连续3天（每小时0.2纳摩尔/毫升），向下丘脑视交叉上核（SCN）上方的第三脑室注入血管活性肠肽（VIP）反义寡脱氧核苷酸，观察其对大鼠脑室内注射2-脱氧-D-葡萄糖（2DG，80微摩尔）引起的高血糖反应的影响。注入VIP反义寡核苷酸后，观察到SCN中VIP表达受到抑制，同时2DG注射所致的高血糖、高胰高血糖素血症和相对低胰岛素血症也受到抑制。此外，额外颅内注射VIP（4纳摩尔）可使接受VIP反义寡核苷酸治疗的大鼠对2DG注射的这些反应恢复。这些发现表明，SCN中的VIP神经元参与了葡萄糖代谢的调节。