Nagata S, Iwasaki K, Kaziro Y
J Biochem. 1976 Jul;80(1):73-7. doi: 10.1093/oxfordjournals.jbchem.a131260.
Since it was shown that the low molecular weight form of the eukaryotic polypeptide chain elongation factor 1 (EF-1alpha) is highly unstable but could be stabilized by the addition of 25% (v/v) glycerol to all the buffer solutions (Nagata, S., Iwasaki, K., & Kaziro, Y. (1976) Arch. Biochem, Biophys, 172, 168-177), its distribution in several tissues has been investigated under conditions where it is stabilized. The results obtained indicate that EF-1alpha is present as a predominant species in all the tissues examined, such as pig liver, rat liver, rabbit reticulocytes, and Artemia salina cysts. Furthermore, it is suggested that the high molecular weight form of the elongation factor 1 contains a new elongation factor, EF-1beta, in addition to EF-1alpha, while the low molecular weight form contains little EF-1beta.
自从有研究表明真核生物多肽链延伸因子1（EF-1α）的低分子量形式高度不稳定，但在所有缓冲溶液中添加25%（v/v）甘油后可使其稳定（长田诚、岩崎和、胜吕义（1976年），《生物化学与生物物理学文献》，172卷，第168 - 177页）以来，人们在其稳定的条件下研究了它在几种组织中的分布情况。所得结果表明，EF-1α在所检测的所有组织中都是主要形式，如猪肝、大鼠肝、兔网织红细胞和卤虫囊肿。此外，有人提出延伸因子1的高分子量形式除了含有EF-1α外，还含有一种新的延伸因子EF-1β，而低分子量形式几乎不含EF-1β。
