肿瘤坏死因子α（TNF-α）调节大鼠肥大细胞反应性。

Tumor necrosis factor alpha (TNF-alpha) modulates rat mast cell reactivity.

作者信息

Olejnik A K, Brzezińska-Błaszczyk E

机构信息

Division of Experimental Immunology, Medical University of Lódz, Poland.

出版信息

Immunol Lett. 1998 Dec;64(2-3):167-71. doi: 10.1016/s0165-2478(98)00106-0.

DOI:10.1016/s0165-2478(98)00106-0

PMID:9870669

Abstract

Nowadays there is growing evidence that some cytokines regulate biological functions of the mature mast cells. Therefore, we have studied whether TNF-alpha, the cytokine of multifunctional activities, could directly stimulate rat peritoneal mast cells to histamine secretion and whether it could modulate rat mast cell reactivity in anaphylactic (with ConA) and anaphylactoid (with compound 48/80) reactions. We have established that rat recombinant TNF-alpha does not activate rat mast cells to histamine release. However, TNF-alpha-treatment causes the decrease of spontaneous histamine release up to 85% (TNF-alpha concentration: 2 x 10(-9) M). Pretreatment of mast cells with TNF-alpha inhibits ConA-stimulate release of histamine with the percent release decreasing up to 33.7% of the control value (TNF-alpha concentration: 5 x 10(-9) M) and this decrease is statistically significant. Pretreatment of mast cells with TNF-alpha reduces compound 48/80-dependent histamine release as well and the percent release of histamine fell to 64.7% of the control value. We have concluded that TNF-alpha may play a significant role in regulation of mast cell secretory activity.

摘要

如今，越来越多的证据表明某些细胞因子可调节成熟肥大细胞的生物学功能。因此，我们研究了具有多种功能的细胞因子肿瘤坏死因子-α（TNF-α）是否能直接刺激大鼠腹膜肥大细胞分泌组胺，以及它是否能在过敏反应（伴刀豆球蛋白A）和类过敏反应（伴化合物48/80）中调节大鼠肥大细胞的反应性。我们发现大鼠重组TNF-α不会激活大鼠肥大细胞释放组胺。然而，TNF-α处理可使组胺的自发释放减少高达85%（TNF-α浓度：2×10⁻⁹ M）。用TNF-α预处理肥大细胞可抑制刀豆球蛋白A刺激的组胺释放，释放百分比降低至对照值的33.7%（TNF-α浓度：5×10⁻⁹ M），且这种降低具有统计学意义。用TNF-α预处理肥大细胞也可减少化合物48/80依赖性组胺释放，组胺释放百分比降至对照值的64.7%。我们得出结论，TNF-α可能在调节肥大细胞分泌活性中发挥重要作用。