Brzezińska-Błaszczyk E, Wyczółkowska J
Arch Immunol Ther Exp (Warsz). 1985;33(4):531-8.
The peritoneal mast cells of rat (Wistar X August) F1 were incubated with purified mouse IgE antibodies and then challenged (in the presence of 50% D2O) with antigen, anti-mouse IgE and concanavalin A. It was found that incubation of mast cells with IgE antibodies led to different level of cell sensitization (in mast cells from different rats), expressed in antigen-induced histamine release (0-52%). Moreover, a) significant antigen-induced histamine release was usually accompanied by high Con A- and anti-IgE-induced histamine release from these cells; the magnitude of release was comparable to Con A- and anti-IgE-induced release from control, nonsensitized cells of the same rat; b) low antigen-induced histamine release was accompanied by the decrease of Con A- and anti-IgE-induced release, as compared to the release from control cells. This fall of reactivity to Con A and anti-IgE was statistically significant and was irreversible during 120 min.
将大鼠（Wistar×August）F1的腹膜肥大细胞与纯化的小鼠IgE抗体一起孵育，然后（在50% D2O存在下）用抗原、抗小鼠IgE和伴刀豆球蛋白A进行激发。发现肥大细胞与IgE抗体孵育导致不同程度的细胞致敏（在来自不同大鼠的肥大细胞中），表现为抗原诱导的组胺释放（0 - 52%）。此外，a）显著的抗原诱导组胺释放通常伴随着这些细胞中伴刀豆球蛋白A和抗IgE诱导的组胺大量释放；释放量与来自同一只大鼠的对照未致敏细胞中伴刀豆球蛋白A和抗IgE诱导的释放量相当；b）与对照细胞的释放相比，低抗原诱导组胺释放伴随着伴刀豆球蛋白A和抗IgE诱导释放的减少。对伴刀豆球蛋白A和抗IgE反应性的这种下降具有统计学意义，并且在120分钟内是不可逆的。
