Fukunaga K, Takada Y, Taniguchi H, Yuzawa K, Otsuka M, Todoroki T, Goto K, Fukao K
Department of Surgery, Institute of Clinical Medicine, Tsukuba University, Tsukuba City, Ibaraki, Japan.
Int Surg. 1998 Jul-Sep;83(3):226-31.
The function of hepatic allograft from non-heartbeating donors (NHBD) is significantly affected by warm ischemic injury before harvesting.
The effects of the endothelin (ET) antagonist TAK-044 and the platelet activating factor (PAF) antagonist E5880 on the function of grafts from NHBD were evaluated in a porcine orthotopic liver transplantation. The liver grafts were subjected to 90 min of warm ischemia and 4 hours cold preservation. Group 1 (n = 4; n is the number of donor/recipient pairs) was used as a control (untreated). In group 2 (n = 4), donors and recipients were treated with TAK-044 (3 mg/kg). In group 3 (n = 4), pigs were treated with E5880 (0.3 mg/kg). In group 4 (n = 4), pigs were treated with TAK-044 and E5880.
The 7 day survival rate of the recipients in groups 1, 2, 3 and 4 was 0, 25, 25 and 100%, respectively (p<0.05, group 1 versus 4). The increases of the serum concentrations of aspartate transaminase, lactate dehydrogenase and arterial lactate 1-4 hours after transplantation were significantly inhibited in the treated groups.
The blockade of ET and PAF has protective effects on the function of hepatic grafts from NHBD.
来自非心脏跳动供体（NHBD）的肝移植移植物的功能受到获取前热缺血损伤的显著影响。
在猪原位肝移植中评估内皮素（ET）拮抗剂TAK - 044和血小板活化因子（PAF）拮抗剂E5880对NHBD移植物功能的影响。肝移植物经历90分钟热缺血和4小时冷保存。第1组（n = 4；n为供体/受体对的数量）用作对照（未治疗）。在第2组（n = 4）中，供体和受体用TAK - 044（3 mg/kg）治疗。在第3组（n = 4）中，猪用E5880（0.3 mg/kg）治疗。在第4组（n = 4）中，猪用TAK - 044和E5880治疗。
第1、2、3和4组受体的7天存活率分别为0、25%、25%和100%（p<0.05，第1组与第4组相比）。治疗组移植后1 - 4小时血清天冬氨酸转氨酶、乳酸脱氢酶浓度及动脉血乳酸的升高受到显著抑制。
ET和PAF的阻断对NHBD肝移植物的功能具有保护作用。
