Kiktenko V S, Balashov N G, Rodina V N
J Hyg Epidemiol Microbiol Immunol. 1976;21(2):207-13.
The semen of animals in spontaneous and experimental leptospirosis was investigated by bacteriological, immunological and biological methods. It has been found that pathogenic leptospirae (L. pomona, L. hebdomadis) are well preserved in the semen and in media used for its dilution. Leptospirae are eliminated with the semen. The initial strain of the agent was isolated from the semen and secretions of 6 out of 26 experimentally infected rabbits and 4 spontaneously recovered bulls (L. hebdomadis). Specific leptospiral antibodies appear in the semen of infected animals. The dynamics of the titre of these antibodies corresponds to the dynamics of the antibody titre in the blood. It is recommended to use the semen for the agglutination reaction. The application of semen infected with pathogenic leptospirae for artificial insemination causes the animals falling ill with leptospirosis accompanied by abortions, reduced fertility and stillbirths of rabbits.
采用细菌学、免疫学和生物学方法对自然感染及实验感染钩端螺旋体病动物的精液进行了研究。结果发现,致病性钩端螺旋体(波摩那群、七日热群)在精液及其稀释所用的培养基中能良好保存。钩端螺旋体随精液排出。从26只实验感染兔中的6只以及4头自然康复公牛(七日热群)的精液和分泌物中分离出了病原体的原始菌株。感染动物的精液中出现了特异性钩端螺旋体抗体。这些抗体滴度的变化动态与血液中抗体滴度的变化动态一致。建议使用精液进行凝集反应。用感染致病性钩端螺旋体的精液进行人工授精会导致动物患钩端螺旋体病,伴有流产、生育力下降和兔死胎。