西西里岛（意大利）流产奶牛中的Sejroe血清群Hardjo血清型：两起牛群病例
Serogroup Sejroe Serovar Hardjo in Aborting Cows: Two Herd Cases in Sicily (Italy).
作者信息
Grippi Francesca, Giudice Elisabetta, Pietro Simona Di, Sciacca Carmela, Santangelo Francesco, Galluzzo Paola, Barreca Santino, Guercio Annalisa
机构信息
Experimental Zooprophylactic Institute of Sicily "A. Mirri", 90129 Palermo, Italy.
Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
出版信息
J Vet Res. 2020 Mar 24;64(1):73-78. doi: 10.2478/jvetres-2020-0021. eCollection 2020 Mar.
INTRODUCTION
The aim of this study was to present two outbreaks of bovine abortion due to infection in cattle herds located in the northern part of Sicily (Italy). The animals were positive for serogroup Sejroe serovar Hardjo in a microscopic agglutination test (MAT).
MATERIAL AND METHODS
A total of 23 Charolaise cows (farm A) and 75 Limousine bulls and Cinisara and Modicana cows (farm B) were enrolled in this study. The blood samples were collected from all subjects at the following time points: before a cycle of intramuscular treatment with oxytetracycline dihydrate (T0), after 5-6 weeks from the treatment (T1), and every 10 weeks until seronegativisation (T2 in Farm A and T3 in Farm B). A serological test (MAT) was used for the diagnosis of leptospirosis.
RESULTS
Two samples from farm A (2/23) and 29 samples from farm B (29/75) were positive to , serogroup Sejroe, serovar Hardjo in the MAT. spp. DNA was detected by real-time PCR in the urine sample of one positive cow on farm A, and in placenta and brain samples belonging to one aborted foetus on farm B.
CONCLUSION
It is important to use serological and molecular diagnostic techniques complementarily to identify infected individuals.
引言
本研究旨在介绍意大利西西里岛北部牛群中因感染导致的两起牛流产疫情。在显微镜凝集试验（MAT）中，这些动物对 sejroe 血清群 hardjo 血清型呈阳性。
材料与方法
本研究共纳入 23 头夏洛来母牛（农场 A）以及 75 头利木赞公牛、奇尼萨拉牛和莫迪卡纳母牛（农场 B）。在以下时间点采集所有受试者的血样：在进行一轮肌肉注射二水四环素治疗前（T0）、治疗后 5 - 6 周（T1），以及每 10 周采集一次直至血清转阴（农场 A 为 T2，农场 B 为 T3）。采用血清学检测（MAT）诊断钩端螺旋体病。
结果
在 MAT 中，农场 A 的 2 份样本（2/23）以及农场 B 的 29 份样本（29/75）对 sejroe 血清群 hardjo 血清型呈阳性。通过实时聚合酶链反应在农场 A 的一头阳性母牛的尿液样本中，以及农场 B 的一头流产胎儿的胎盘和脑样本中检测到了钩端螺旋体属 DNA。
结论
互补使用血清学和分子诊断技术对于识别感染个体很重要。
Zotero 插件