Tapparo A, Satre M, Klein G
Laboratoire de Biochimie et de Biophysique des Systèmes Intégrés (UMR CNRS-CEA 314), Département de Biologie Moléculaire et Structurale, CEA-Grenoble, 17 rue des Martyrs, F-38054 Grenoble cedex 09, France.
Curr Genet. 1998 Dec;34(5):410-8. doi: 10.1007/s002940050415.
The sequences of the rps4 and rps10 genes encoding the Dictyostelium discoideum homologues of the basic ribosomal proteins S4 and S10 were determined from cDNA and genomic DNA clones. They are expressed respectively as 266 and 153 amino-acid-long proteins. In both cases, the N-terminal methionine is cleaved in the mature proteins. S4 contains two putative nuclear targeting signals and displays a strong overall identity (around 60%) to eukaryotic S4 homologues. The rps10 gene harbours a 314-bp intron located close to its 5'-coding end. The overall identity between D. discoideum S10 and eukaryotic homologues is around 38% and rises to 53% in the N-terminal domain. Southern blots suggest that both S4 and S10 are encoded by single genes that are regulated during development. The corresponding mRNAs decrease sharply after 8 h of differentiation.
从cDNA和基因组DNA克隆中确定了编码盘基网柄菌基本核糖体蛋白S4和S10同源物的rps4和rps10基因的序列。它们分别表达为长度为266和153个氨基酸的蛋白质。在这两种情况下，成熟蛋白中的N端甲硫氨酸都会被切除。S4包含两个假定的核靶向信号，并且与真核生物S4同源物具有很强的整体同一性（约60%）。rps10基因在其5'编码端附近有一个314 bp的内含子。盘基网柄菌S10与真核生物同源物之间的整体同一性约为38%，在N端结构域中升至53%。Southern印迹表明，S4和S10均由单个基因编码，这些基因在发育过程中受到调控。分化8小时后，相应的mRNA急剧减少。
