Shimizu S, Mori H
Graduate School of Bioagricultural Science, Nagoya University, Japan.
Plant Cell Physiol. 1998 Oct;39(10):1073-9. doi: 10.1093/oxfordjournals.pcp.a029304.
Apical dominance is a phenomenon in which a terminal bud grows predominantly and the growth of the axillary buds is suppressed. Here, we investigated the molecular mechanisms associated with cell cycle control that occur in pea axillary buds as a result of decapitation. Proliferating cell nuclear antigen (PCNA) protein was detected in both dormant and growing buds, while PCNA mRNA was absent in dormant buds. Pissa; CycB1;2 and Cdc2 proteins were undetectable during dormancy. To analyze an interaction between PCNA and Pissa;CycD3;1, we performed anti-PCNA immunoaffinity column chromatography. Pissa;CycD3;1 protein was detected in the eluate prepared from the dormant buds, but not in the eluate prepared from the growing buds. Furthermore, we performed anti-Pissa;CycD3;1 immunoaffinity column chromatography. PCNA protein was detected in the eluate prepared from the dormant buds, but not in the eluate prepared from the growing buds. These results indicated that PCNA associated with Pissa;CycD3;1 only during dormancy. In addition, the interaction between PCNA and Pissa;CycD3;1 was confirmed by a yeast two-hybrid system.
顶端优势是一种顶芽优先生长而腋芽生长受抑制的现象。在此，我们研究了豌豆腋芽因去顶而发生的与细胞周期调控相关的分子机制。在休眠芽和生长芽中均检测到增殖细胞核抗原（PCNA）蛋白，而休眠芽中不存在PCNA mRNA。在休眠期间未检测到Pissa; CycB1;2和Cdc2蛋白。为了分析PCNA与Pissa; CycD3;1之间的相互作用，我们进行了抗PCNA免疫亲和柱层析。在从休眠芽制备的洗脱液中检测到Pissa; CycD3;1蛋白，但在从生长芽制备的洗脱液中未检测到。此外，我们进行了抗Pissa; CycD3;1免疫亲和柱层析。在从休眠芽制备的洗脱液中检测到PCNA蛋白，但在从生长芽制备的洗脱液中未检测到。这些结果表明PCNA仅在休眠期间与Pissa; CycD3;1相关联。此外，通过酵母双杂交系统证实了PCNA与Pissa; CycD3;1之间的相互作用。
