Stafstrom J P, Ripley B D, Devitt M L, Drake B
Department of Biological Sciences, Northern Illinois University 60115, USA.
Planta. 1998 Aug;205(4):547-52. doi: 10.1007/s004250050354.
Pea (Pisum sativum L. cv. Alaska) axillary buds can be stimulated to cycle between dormant and growing states. Dormant buds synthesize unique proteins and are as metabolically active as growing buds. Two cDNAs, PsDRM1 and PsDRM2, were isolated from a dormant bud library. The deduced amino acid sequence of PsDRM1 (111 residues) is 75% identical to that of an auxin-repressed strawberry clone. PsDRM2 encodes a putative protein containing 129 residues, which includes 11 repeats of the sequence [G]-GGGY[H][N] (the bracketed residues may be absent). PsDRM2 is related to cold- and ABA-stimulated clones from alfalfa. Decapitating the terminal bud rapidly stimulates dormant axillary buds to begin growing. The abundance of PsDRM1 mRNA in axillary buds declines 20-fold within 6 h of decapitation; it quickly reaccumulates when buds become dormant again. The level of PsDRM2 mRNA is about three fold lower in growing buds than in dormant buds. Expression of PsDRM1 is enhanced in other non-growing organs (roots >> root apices; fully-elongated stems > elongating stems), and thus is an excellent "dormancy" marker. In contrast, PsDRM2 expression is not dormancy-associated in other organs.
豌豆（Pisum sativum L. cv. Alaska）的腋芽能够被刺激在休眠状态和生长状态之间循环。休眠芽会合成独特的蛋白质，并且其代谢活性与生长芽一样高。从一个休眠芽文库中分离出了两个cDNA，即PsDRM1和PsDRM2。PsDRM1推导的氨基酸序列（111个残基）与一个生长素抑制的草莓克隆的氨基酸序列有75%的同一性。PsDRM2编码一个含有129个残基的推定蛋白质，其中包括序列[G]-GGGY[H][N]的11个重复（方括号内的残基可能不存在）。PsDRM2与来自苜蓿的受低温和脱落酸刺激的克隆相关。去除顶芽会迅速刺激休眠的腋芽开始生长。在去除顶芽后的6小时内，腋芽中PsDRM1 mRNA的丰度下降20倍；当芽再次进入休眠状态时，它会迅速重新积累。生长芽中PsDRM2 mRNA的水平比休眠芽中低约三倍。PsDRM1在其他非生长器官（根>>根尖；完全伸长的茎>正在伸长茎）中的表达增强，因此是一个出色的“休眠”标记。相比之下，PsDRM2在其他器官中的表达与休眠无关。
