Sawa M, Imaeda Y, Hiratake J, Fujii R, Umeshita R, Watanabe M, Kondo H, Oda J
Institute for Chemical Research, Kyoto University, Japan.
Bioorg Med Chem Lett. 1998 Mar 17;8(6):647-52. doi: 10.1016/s0960-894x(98)00080-8.
Hapten 4 was synthesized to generate catalytic antibodies triggering chemiluminescence by catalyzing the decomposition of the 1,2-dioxetane 3. The hapten 4 was so designed as to elicit a negatively charged functional group in the antibody combining site to catalyze the beta-elimination of the protecting group in 3 as well as to lock the protecting group into an energetically favorable anti-periplanar conformation.
合成了半抗原4，以产生通过催化1,2 - 二氧杂环丁烷3的分解来触发化学发光的催化抗体。半抗原4的设计目的是在抗体结合位点引发一个带负电荷的官能团，以催化3中保护基团的β - 消除反应，并将保护基团锁定在能量上有利的反式共平面构象中。
