Heurtin-Le Corre C, Donnio P Y, Bonnier M, Travert M F, Lacourt A, Avril J L
Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Rennes, France.
Pathol Biol (Paris). 1998 Oct;46(8):587-90.
To determine the prevalence of quinolone resistance in Salmonella typhimurium strains from humans or animals (cattle, poultry, swine), the S. typhimurium strains isolated at a teaching hospital and at the central veterinary laboratory of the same district between January 1, 1995, and December 31, 1996 were studied. Susceptibility to nalidixic acid was determined using the disk diffusion method. Strains with decreased susceptibility to nalidixic acid were subjected to minimal inhibitory concentration (MIC) determination for pefloxacin, ofloxacin, ciprofloxacin, norfloxacin, levofloxacin, and grepafloxacin. Decreased susceptibility to nalidixic acid was demonstrated for 41 of the 309 strains studied and increased from 8.5% in 1995 to 18.6% in 1996. MIC90 values of fluoroquinolones for strains with decreased susceptibility to nalidixic acid were lower than 1 mg/L, which is the cutoff above which a strain is classified as susceptible, but were higher than for strains that were susceptible to nalidixic acid. These low levels of resistance may be the first step in selection of mutant strains with high levels of resistance to fluoroquinolones. This warrants continued monitoring of resistance of Salmonella to fluoroquinolones.
为确定来自人类或动物（牛、家禽、猪）的鼠伤寒沙门氏菌菌株对喹诺酮类药物的耐药率，我们对1995年1月1日至1996年12月31日期间在一家教学医院和同一地区的中央兽医实验室分离出的鼠伤寒沙门氏菌菌株进行了研究。采用纸片扩散法测定对萘啶酸的敏感性。对萘啶酸敏感性降低的菌株进行了对培氟沙星、氧氟沙星、环丙沙星、诺氟沙星、左氧氟沙星和格帕沙星的最低抑菌浓度（MIC）测定。在所研究的309株菌株中，有41株对萘啶酸的敏感性降低，且从1995年的8.5%增至1996年的18.6%。对萘啶酸敏感性降低的菌株的氟喹诺酮类药物MIC90值低于1mg/L（该浓度以上的菌株被分类为敏感），但高于对萘啶酸敏感的菌株。这些低水平耐药可能是选择对氟喹诺酮类药物具有高水平耐药性的突变菌株的第一步。这就需要持续监测沙门氏菌对氟喹诺酮类药物的耐药情况。
