Devallois A, Horgen L, Sola C, Rastogi N
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Pointe-à-Pitre, France.
Pathol Biol (Paris). 1998 Oct;46(8):625-36.
Molecular typing is now widely used in mycobacteriology as a significant addition to conventional epidemiological studies. Several methods are available that differ with respect to their discriminating power and to the genetic marker used. Restriction fragment length polymorphism (RFLP) done using the IS6110 insertion sequence has been widely used for typing Mycobacterium tuberculosis. More recently, short repetitive DNA sequences have been used as RFLP markers, as well as for other typing methods involving gene amplification. One of these methods, the inter-direct repeat (DR)-based spoligotyping method, is rapid, directly applicable to clinical specimens, and highly discriminating.
分子分型目前在分枝杆菌学中被广泛应用，是对传统流行病学研究的重要补充。有几种方法可供使用，它们在鉴别能力和所使用的遗传标记方面有所不同。使用IS6110插入序列进行的限制性片段长度多态性（RFLP）分析已被广泛用于结核分枝杆菌的分型。最近，短重复DNA序列已被用作RFLP标记，以及用于其他涉及基因扩增的分型方法。其中一种方法，即基于间隔直接重复序列（DR）的spoligotyping方法，快速、可直接应用于临床标本，且鉴别力高。
