Igarashi T, Yamamoto A, Goto N
Department of Oral Microbiology, Showa University School of Dentistry, Tokyo, Japan.
Oral Microbiol Immunol. 1998 Dec;13(6):382-6. doi: 10.1111/j.1399-302x.1998.tb00696.x.
Thirty-one strains of 23 gram-negative oral bacterial species were examined for dextran-degrading activity on agar plates containing blue dextran. One strain each of Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella loescheii, Prevotella melaninogenica and Prevotella oralis had detectable dextranase activity. The culture supernatants of P. melaninogenica and P. oralis cells contained dextranases of multiple sizes, but those of the other three species had a single size of enzyme. A 56-kDa dextranase was purified from the culture supernatant of P. oralis and the antiserum against the enzyme was prepared with a rabbit. The Ouchterlony test showed that the antibody reacted with the supernatants of both P. melaninogenica and P. oralis but not with the others. Dot-blot hybridization using the dextranase gene of Streptococcus mutans as a probe revealed that there was no significantly homologous sequence in the chromosomal DNA of the five species.
对23种革兰氏阴性口腔细菌的31个菌株在含有蓝色葡聚糖的琼脂平板上进行葡聚糖降解活性检测。赭色二氧化碳嗜纤维菌、生痰二氧化碳嗜纤维菌、洛氏普雷沃菌、产黑色素普雷沃菌和口腔普雷沃菌各有1个菌株具有可检测到的葡聚糖酶活性。产黑色素普雷沃菌和口腔普雷沃菌细胞的培养上清液含有多种大小的葡聚糖酶，而其他三个菌种的培养上清液中葡聚糖酶只有单一大小。从口腔普雷沃菌的培养上清液中纯化出一种56 kDa的葡聚糖酶，并用兔子制备了针对该酶的抗血清。免疫双扩散试验表明，该抗体与产黑色素普雷沃菌和口腔普雷沃菌的上清液发生反应，但与其他菌的上清液不发生反应。以变形链球菌的葡聚糖酶基因作为探针进行斑点印迹杂交，结果显示这五个菌种的染色体DNA中不存在明显的同源序列。
