Mastrocola T, Porcelli A M, Rugolo M
Dipartimento di Biologia Ev.Sp., Università di Bologna, Italy.
FEBS Lett. 1998 Dec 4;440(3):268-72. doi: 10.1016/s0014-5793(98)01468-9.
C127 cell lines transfected with wtCFTR, delta F508CFTR or vector were employed to determine HCO3- fluxes in the presence or absence of functional CFTR, using the pH-sensitive dye BCECF. Both cytosolic alkalinization and acidification were due to activity of anion exchanger and were similar in the three cell lines, indicating that expression of CFTR did not influence anion exchanger activity. In C127wt cells only, cAMP elevating agents significantly stimulated HCO3- fluxes, insensitive to the inhibitor of anion exchanger 4,4'-diisothiocyanate dihydrostilbene-2,2'-disulfonic acid, suggesting that activated CFTR directly mediates both HCO3- influx and efflux and therefore can contribute to intracellular and extracellular pH regulation.
使用对pH敏感的染料BCECF，对转染了野生型CFTR、ΔF508CFTR或载体的C127细胞系进行研究，以测定在有或没有功能性CFTR的情况下的HCO₃⁻通量。胞质碱化和酸化均归因于阴离子交换器的活性，并且在这三种细胞系中相似，这表明CFTR的表达不影响阴离子交换器的活性。仅在C127wt细胞中，cAMP升高剂显著刺激HCO₃⁻通量，且对阴离子交换器抑制剂4,4'-二异硫氰酸二氢芪-2,2'-二磺酸不敏感，这表明活化的CFTR直接介导HCO₃⁻内流和外流，因此可有助于细胞内和细胞外pH调节。
