Hagedoorn P L, Driessen M C, van den Bosch M, Landa I, Hagen W R
Wageningen University and Research Centre, Department of Biomolecular Sciences, The Netherlands.
FEBS Lett. 1998 Dec 4;440(3):311-4. doi: 10.1016/s0014-5793(98)01466-5.
The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The Ems of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the Em: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution.
嗜热栖热菌红素氧化还原蛋白和铁氧化还原蛋白的氧化还原化学性质已在直接伏安法以及电子顺磁共振（EPR）监测的整体滴定法中作为温度的函数进行了研究。通过直接伏安法测量的两种蛋白质的标准氧化还原电位（Em）具有正常（线性）的温度依赖性，且无pH依赖性。EPR监测不是确定Em温度依赖性的可靠方法：蛋白质在快速冷冻时会呈现出与溶液冰点相对应的构象。
