Fleischmann M, Bloch W, Kolossov E, Andressen C, Müller M, Brem G, Hescheler J, Addicks K, Fleischmann B K
Department of Biotechnology in Animal Production, Interuniversitäres Forschungsinstitut für Agrarbiotechnologie, Tulln, Austria.
FEBS Lett. 1998 Dec 4;440(3):370-6. doi: 10.1016/s0014-5793(98)01476-8.
We demonstrate the establishment of transgenic mice, where the expression of the green fluorescent protein (GFP) is under control of the human cardiac alpha-actin promoter. These mice display cardiac specific GFP expression already during early embryonic development. Prominent GFP fluorescence was observed at the earliest stage of the murine heart anlage (E8). Cardiomyocytes of different developmental stages proved GFP positive, but the intensity varied between cells. We further show that contractions of single GFP positive cardiomyocytes can be monitored within the intact embryo. At later stages of embryonic development, the skeletal musculature was also GFP positive, in line with the known expression pattern of cardiac alpha-actin. The tissue specific labeling of organs is a powerful new tool for embryological as well as functional investigations in vivo.
我们展示了转基因小鼠的建立，其中绿色荧光蛋白（GFP）的表达受人类心脏α-肌动蛋白启动子的控制。这些小鼠在胚胎发育早期就已表现出心脏特异性GFP表达。在小鼠心脏原基的最早阶段（E8）就观察到了明显的GFP荧光。不同发育阶段的心肌细胞被证明为GFP阳性，但细胞之间的强度有所不同。我们进一步表明，可以在完整胚胎内监测单个GFP阳性心肌细胞的收缩。在胚胎发育的后期阶段，骨骼肌组织也呈GFP阳性，这与心脏α-肌动蛋白的已知表达模式一致。器官的组织特异性标记是体内胚胎学以及功能研究的强大新工具。
