Choi Min Ah, Ahn Geum Ran, Kim Seong Hwan
Department of Microbiology, Dankook University, Cheonan, Korea.
Mycobiology. 2019 Dec 26;47(4):430-440. doi: 10.1080/12298093.2019.1703529. eCollection 2019.
Fungal contamination of built-in furniture is a frequent problem in Korea when new apartment is built. However, domestic information on the contaminating fungi is very limited. This study was conducted to isolate, identify and characterize the fungi of the problem in one of the apartment houses where the fungi were claimed in the built-in furniture before the house owner moves in. Fungi present in the furniture installed in a main room, dress room, and kitchen side were visually and microscopically confirmed and purely isolated on PDA. The isolated fungi were identified by analyzing the morphological characteristics and nucleotide sequence of the ITS, calmodulin gene, and TEF-1α gene. , , , , and were identified. Four out of the six fungal species were positive for at least one enzyme in six kinds of extracellular enzyme assays. When these four species ( and ) were inoculated onto four kinds of wood chips of furniture materials, they were able to colonize all of the wood chips. Their settlement was better at 95% humidity condition than at 30% humidity condition. Among the four species, caused the darkest discoloration and secreted the most number of extracellular enzymes. The four species were re-isolated from the colonized wood chips and confirmed as the problematic fungi in the built-in furniture.
在韩国，新建公寓时内置家具的真菌污染是一个常见问题。然而，关于污染真菌的国内信息非常有限。本研究旨在对一户公寓楼中出现问题的真菌进行分离、鉴定和特征描述，该公寓楼在房主入住前，内置家具中就发现了真菌。对安装在主房间、更衣室和厨房的家具中的真菌进行了肉眼和显微镜确认，并在马铃薯葡萄糖琼脂（PDA）上进行了纯培养分离。通过分析核糖体DNA内转录间隔区（ITS）、钙调蛋白基因和延伸因子-1α（TEF-1α）基因的形态特征和核苷酸序列，对分离出的真菌进行了鉴定。鉴定出了[具体真菌名称1]、[具体真菌名称2]、[具体真菌名称3]、[具体真菌名称4]、[具体真菌名称5]和[具体真菌名称6]。在六种胞外酶测定中，六种真菌中有四种至少对一种酶呈阳性。当将这四种真菌（[具体真菌名称1]和[具体真菌名称2]）接种到四种家具材料的木片上时，它们能够在所有木片上定殖。它们在95%湿度条件下的定殖情况比在30%湿度条件下更好。在这四种真菌中，[具体真菌名称3]导致的变色最深，分泌的胞外酶数量最多。从定殖的木片中重新分离出这四种真菌，并确认为内置家具中的问题真菌。
