用于检测接种和自然感染鳟鱼组织中鲁氏耶尔森菌的聚合酶链反应检测方法的开发。
Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout.
作者信息
Gibello A, Blanco M M, Moreno M A, Cutuli M T, Domenech A, Domínguez L, Fernández-Garayzábal J F
机构信息
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
出版信息
Appl Environ Microbiol. 1999 Jan;65(1):346-50. doi: 10.1128/AEM.65.1.346-350.1999.
Abstract
A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues.
摘要
开发了一种基于聚合酶链反应（PCR）的方法，用于特异性检测接种的鳟鱼组织和自然感染的鳟鱼组织中的鲁氏耶尔森菌。对其他耶尔森菌属细菌、鱼类细菌性病原菌或系统发育相关细菌（n = 34）进行检测时，未获得扩增产物。PCR检测的灵敏度为每个PCR管60至65个细菌细胞，通过与非放射性探针杂交，灵敏度可降至10至20个细胞。对于检测感染鳟鱼组织中的鲁氏耶尔森菌，PCR检测方法被证明与传统培养方法一样可靠，且速度更快。
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