用于快速灵敏检测鱼类、沉积物和水中创伤弧菌的巢式聚合酶链反应方法。
Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.
作者信息
Arias C R, Garay E, Aznar R
机构信息
Departamento de Microbiología, Universitat de Valencia, Burjassot, Spain.
出版信息
Appl Environ Microbiol. 1995 Sep;61(9):3476-8. doi: 10.1128/aem.61.9.3476-3478.1995.
Abstract
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
摘要
开发了一种用于检测养鱼场中创伤弧菌的巢式PCR，作为培养方法的替代方法，通过使用针对23S rRNA基因的创伤弧菌特异性序列两侧的通用引物。这种特异性检测方法能够在不进行富集的情况下，在24小时内检测出人工接种样品中10 fg的DNA或12至120个细胞。
相似文献
1
Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.用于快速灵敏检测鱼类、沉积物和水中创伤弧菌的巢式聚合酶链反应方法。
Appl Environ Microbiol. 1995 Sep;61(9):3476-8. doi: 10.1128/aem.61.9.3476-3478.1995.
2
Rapid and sensitive PCR detection of Vibrio trachuri pathogenic to Japanese horse mackerel (Trachurus japonicus).
Microbiol Immunol. 1995;39(12):1003-6. doi: 10.1111/j.1348-0421.1995.tb03290.x.
3
A comparison of strategies for the detection and recovery of Vibrio vulnificus from marine samples of the western Mediterranean coast.西地中海沿岸海洋样本中创伤弧菌检测与复苏策略的比较
Syst Appl Microbiol. 1998 Mar;21(1):128-34. doi: 10.1016/S0723-2020(98)80016-7.
4
Analysis of the 16S-23S rDNA intergenic spacers (IGSs) of marine vibrios for species-specific signature DNA sequences.分析海洋弧菌的16S-23S核糖体DNA间隔区（IGS）以寻找物种特异性特征DNA序列。
Mar Pollut Bull. 2002 May;44(5):412-20. doi: 10.1016/s0025-326x(01)00256-9.
5
Evaluation of the polymerase chain reaction method for identification of Vibrio vulnificus isolated from marine environments.
J Food Prot. 1997 Jan;60(1):81-3. doi: 10.4315/0362-028x-60.1.81.
6
Sequence determination of rRNA genes of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes.致病性弧菌属物种rRNA基因的序列测定及用rRNA靶向寡核苷酸探针进行创伤弧菌的全细胞鉴定
Int J Syst Bacteriol. 1994 Apr;44(2):330-7. doi: 10.1099/00207713-44-2-330.
7
Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR).通过多重聚合酶链反应（PCR）检测海洋致病性弧菌属细菌
J Environ Biol. 2008 Jul;29(4):543-6.
8
PCR and molecular detection for differentiating Vibrio species.用于区分弧菌属菌种的聚合酶链反应及分子检测
Ann N Y Acad Sci. 2002 Oct;969:60-5. doi: 10.1111/j.1749-6632.2002.tb04351.x.
9
Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.通过聚合酶链反应扩增检测鳗鱼和牡蛎中的创伤弧菌生物型1和生物型2
Appl Environ Microbiol. 1996 Apr;62(4):1378-82. doi: 10.1128/aem.62.4.1378-1382.1996.
10
Sequence of a cloned pR72H fragment and its use for detection of Vibrio parahaemolyticus in shellfish with the PCR.克隆的pR72H片段的序列及其在利用聚合酶链式反应检测贝类中副溶血性弧菌的应用
Appl Environ Microbiol. 1995 Apr;61(4):1311-7. doi: 10.1128/aem.61.4.1311-1317.1995.
引用本文的文献
1
Species in Wastewater Final Effluents and Receiving Watershed in South Africa: Implications for Public Health.南非污水最终排放和受纳水域中的物种：对公共健康的影响。
Int J Environ Res Public Health. 2018 Jun 15;15(6):1266. doi: 10.3390/ijerph15061266.
2
Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species.与人类致病弧菌属相关的深海热液喷口细菌。
Proc Natl Acad Sci U S A. 2015 May 26;112(21):E2813-9. doi: 10.1073/pnas.1503928112. Epub 2015 May 11.
3
Diversity and impact of prokaryotic toxins on aquatic environments: a review.原核生物毒素的多样性及其对水生环境的影响：综述。
Toxins (Basel). 2010 Oct;2(10):2359-410. doi: 10.3390/toxins2102359. Epub 2010 Oct 18.
4
Detection of Batrachochytrium dendrobatidis in endemic salamander species from central Texas.检测来自德克萨斯州中部的地方性蝾螈物种中的蛙壶菌。
Ecohealth. 2009 Mar;6(1):20-6. doi: 10.1007/s10393-009-0229-x. Epub 2009 May 8.
5
Comparison of conventional, nested, and real-time PCR assays for rapid and accurate detection of Vibrio vulnificus.用于快速准确检测创伤弧菌的常规PCR、巢式PCR和实时PCR检测方法的比较
J Clin Microbiol. 2008 Sep;46(9):2992-8. doi: 10.1128/JCM.00027-08. Epub 2008 Jul 9.
6
Biodiversity of vibrios.弧菌的生物多样性。
Microbiol Mol Biol Rev. 2004 Sep;68(3):403-31, table of contents. doi: 10.1128/MMBR.68.3.403-431.2004.
7
Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray.利用多重PCR和DNA微阵列同时检测海洋鱼类病原体。
J Clin Microbiol. 2004 Apr;42(4):1414-9. doi: 10.1128/JCM.42.4.1414-1419.2004.
8
Rapid detection of Vibrio vulnificus in shellfish and Gulf of Mexico water by real-time PCR.通过实时聚合酶链反应快速检测贝类和墨西哥湾水中的创伤弧菌
Appl Environ Microbiol. 2004 Jan;70(1):498-507. doi: 10.1128/AEM.70.1.498-507.2004.
9
Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater.在人工海水中环境及临床创伤弧菌菌株的不可培养群体中检测细胞毒素-溶血素mRNA
Appl Environ Microbiol. 2002 Nov;68(11):5641-6. doi: 10.1128/AEM.68.11.5641-5646.2002.
10
A selective medium and a specific probe for detection of Vibrio vulnificus.一种用于检测创伤弧菌的选择性培养基和特异性探针。
Appl Environ Microbiol. 2000 Feb;66(2):855-9. doi: 10.1128/AEM.66.2.855-859.2000.
本文引用的文献
1
Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.用于检测食品中霍乱弧菌的快速聚合酶链反应方法。
Appl Environ Microbiol. 1993 Feb;59(2):556-60. doi: 10.1128/aem.59.2.556-560.1993.
2
Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe.使用碱性磷酸酶标记的寡核苷酸探针在非选择性培养基上快速鉴定创伤弧菌。
Appl Environ Microbiol. 1993 Feb;59(2):541-6. doi: 10.1128/aem.59.2.541-546.1993.
3
Sequence determination of rRNA genes of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes.致病性弧菌属物种rRNA基因的序列测定及用rRNA靶向寡核苷酸探针进行创伤弧菌的全细胞鉴定
Int J Syst Bacteriol. 1994 Apr;44(2):330-7. doi: 10.1099/00207713-44-2-330.
4
A PCR-based assay for the identification of the fish pathogen Renibacterium salmoninarum.一种基于聚合酶链式反应（PCR）的检测方法，用于鉴定鱼类病原体鲑鱼肾杆菌。
FEMS Microbiol Lett. 1994 Jan 15;115(2-3):131-6. doi: 10.1111/j.1574-6968.1994.tb06627.x.
5
Detection of Aeromonas salmonicida, causal agent of furunculosis in salmonid fish, from the tank effluent of hatchery-reared Atlantic salmon smolts.从孵化场养殖的大西洋鲑鱼苗种养殖池废水中检测杀鲑气单胞菌，它是鲑科鱼类疖疮病的病原体。
Appl Environ Microbiol. 1994 Oct;60(10):3874-7. doi: 10.1128/aem.60.10.3874-3877.1994.
6
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.大肠杆菌核糖体RNA操纵子的基因组织与一级结构
J Mol Biol. 1981 May 15;148(2):107-27. doi: 10.1016/0022-2836(81)90508-8.
7
Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters.
Appl Environ Microbiol. 1991 Mar;57(3):707-11. doi: 10.1128/aem.57.3.707-711.1991.
8
The risk of Vibrio illness in the Florida raw oyster eating population, 1981-1988.
Am J Epidemiol. 1991 Aug 1;134(3):290-7. doi: 10.1093/oxfordjournals.aje.a116084.
9
Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells.聚合酶链反应在检测可培养和不可培养的创伤弧菌细胞中的应用。
Appl Environ Microbiol. 1991 Sep;57(9):2651-5. doi: 10.1128/aem.57.9.2651-2655.1991.
10
Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state.创伤弧菌不可培养细胞的形成及其与饥饿状态的关系。
Appl Environ Microbiol. 1991 Sep;57(9):2640-4. doi: 10.1128/aem.57.9.2640-2644.1991.
Zotero 插件