一种用于固相合成具有N端半胱氨酸的肽核酸的简单方法。

A simple procedure for solid-phase synthesis of peptide nucleic acids with N-terminal cysteine.

作者信息

Goodwin T E, Holland R D, Lay J O, Raney K D

机构信息

Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.

出版信息

Bioorg Med Chem Lett. 1998 Aug 18;8(16):2231-4. doi: 10.1016/s0960-894x(98)00400-4.

DOI:10.1016/s0960-894x(98)00400-4

PMID:9873519

Abstract

Problems were encountered during attempts to prepare N-terminal cysteine-substituted peptide nucleic acids (PNAs) from commercially available, Fmoc-protected monomers. These problems have been surmounted by the use of an S-t-butylmercapto protecting group on the cysteine moiety. The solid-phase syntheses are carried out via a simplified procedure which should be generally useful for manual PNA synthesis.

摘要

在尝试从市售的Fmoc保护的单体制备N端半胱氨酸取代的肽核酸（PNA）的过程中遇到了问题。通过在半胱氨酸部分使用S-叔丁基巯基保护基团克服了这些问题。固相合成通过简化的程序进行，该程序通常可用于手动PNA合成。