热稳定法呢基二磷酸合酶对异戊烯基二磷酸烷基同系物的底物特异性。

Substrate specificity of thermostable farnesyl diphosphate synthase with alkyl group homologs of isopentenyl diphosphate.

作者信息

Nagaki M, Kannari H, Ishibashi J, Maki Y, Nishino T, Ogura K, Koyama T

机构信息

Department of Materials Science and Technology, Faculty of Science and Technology, Hirosaki University, Aomori, Japan.

出版信息

Bioorg Med Chem Lett. 1998 Sep 22;8(18):2549-54. doi: 10.1016/s0960-894x(98)00458-2.

DOI:10.1016/s0960-894x(98)00458-2

PMID:9873578

Abstract

3-Alkyl group homologs of isopentenyl diphosphate were examined for the reactivity as substrates of the thermostable farnesyl diphosphate (FPP) synthase of Bacillus stearothermophilus. Even 3-n-propyl- and 3-n-butyl-but-3-enyl diphosphates, which are hardly acceptable by animal FPP synthases, are accepted by this bacterial enzyme as substrates to react with dimethylallyl- and geranyl diphosphates, yielding 7-methyl-3-n-propylocta-2,6-dienyl- and 7,11-dimethyl-3-n-propyldodeca-2,6,10-trienyl diphosphate, respectively.

摘要

研究了异戊烯基二磷酸的3-烷基同系物作为嗜热脂肪芽孢杆菌耐热法呢基二磷酸（FPP）合酶底物的反应活性。即使是动物FPP合酶几乎不接受的3-正丙基-和3-正丁基-3-丁烯基二磷酸，这种细菌酶也能将其作为底物与二甲基烯丙基二磷酸和香叶基二磷酸反应，分别生成7-甲基-3-正丙基辛-2,6-二烯基二磷酸和7,11-二甲基-3-正丙基十二-2,6,10-三烯基二磷酸。