在缺乏法尼基二磷酸合酶的ispA缺失大肠杆菌菌株中，将异戊烯基二磷酸与二甲基烯丙基二磷酸缩合的短链异戊烯基二磷酸合酶。
Short-chain prenyl diphosphate synthase that condenses isopentenyl diphosphate with dimethylallyl diphosphate in ispA null Escherichia coli strain lacking farnesyl diphosphate synthase.
作者信息
Saito Ken, Fujisaki Shingo, Nishino Tokuzo
机构信息
Department of Bioengineering, Faculty of Engineering, Tohoku University, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
出版信息
J Biosci Bioeng. 2007 Jun;103(6):575-7. doi: 10.1263/jbb.103.575.
A short-chain prenyl diphosphate synthase in an Escherichia coli mutant that lacked the gene coding for farnesyl diphosphate synthase, ispA, was separated from other prenyl diphosphate synthases by DEAE-Toyopearl column chromatography. The purified enzyme catalyzed the condensation of isopentenyl diphosphate with dimethylallyl diphosphate to form farnesyl diphosphate and geranylgeranyl diphosphate.
在缺乏编码法呢基二磷酸合酶（ispA）基因的大肠杆菌突变体中，一种短链异戊烯基二磷酸合酶通过DEAE- Toyopearl柱色谱法与其他异戊烯基二磷酸合酶分离。纯化后的酶催化异戊烯基二磷酸与二甲基烯丙基二磷酸缩合形成法呢基二磷酸和香叶基香叶基二磷酸。
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