胰腺囊肿液中pS2蛋白的测定。pS2蛋白在黏液性囊性肿瘤发病机制中潜在作用的证据。

Measurement of pS2 protein in pancreatic cyst fluids. Evidence for a potential role of pS2 protein in the pathogenesis of mucinous cystic tumors.

作者信息

Yang J M, Lee J, Southern J F, Warshaw A L, Dhanak E, Lewandrowski K B

机构信息

Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, USA.

出版信息

Int J Pancreatol. 1998 Dec;24(3):181-6. doi: 10.1007/BF02788420.

DOI:10.1007/BF02788420

PMID:9873952

Abstract

CONCLUSION

Elevated levels of the growth factor pS2 protein in the cyst fluids of mucinous cystic tumors correlate with earlier observations using immunohistochemical techniques showing that pS2 protein is expressed by these tumors. The markedly elevated levels of pS2 protein compared to normal plasma values suggest that this growth factor may be important in the pathogenesis of pancreatic mucinous cystic tumors.

BACKGROUND

Cystic lesions of the pancreas include inflammatory pseudocysts, serous cystadenomas, and mucinous cystic tumors, some of which are malignant. Previous studies using immunohistochemical techniques have shown that virtually all pancreatic mucinous tumors express pS2 protein. pS2 protein is a growth factor that is believed to be important in the normal process of inflammation and repair. We measured pS2 protein and other growth factors in pancreatic cyst fluids to assess their potential pathophysiologic and diagnostic significance.

METHODS

Levels of pS2 protein were measured in 54 pancreatic cyst fluids by radioimmunoassay. The growth factors, epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), and insulin-like growth factors I and II (IGF-I, IGF-II) were measured in 22 cyst fluids using commercial immunoassays.

RESULTS

Mucinous cysts exhibited significantly higher levels of cyst fluid pS2 protein than nonmucinous lesions, including pseudocysts and serous cystadenomas (median: 78,303 pg/mL; range: 218-361,176 pg/mL vs median: 886 pg/mL; range: 0-14,206 pg/mL; p = 0.0001). The level of pS2 in mucinous tumors was markedly higher than plasma values (median: 392 pg/mL). Levels of pS2 protein in malignant mucinous lesions tended to be higher than those in benign mucinous cysts, but this difference was not statistically significant (median: 88,817 vs 64,350 pg/mL; p = 0.159). Levels of other growth factors, including EGF, TGF-alpha, IGF-I, and IGF-II, did not discriminate among the different cyst types, and the values were within normal plasma ranges.

摘要

结论

黏液性囊性肿瘤囊液中生长因子pS2蛋白水平升高，这与早期使用免疫组化技术的观察结果相关，该技术显示这些肿瘤表达pS2蛋白。与正常血浆值相比，pS2蛋白水平显著升高表明该生长因子可能在胰腺黏液性囊性肿瘤的发病机制中起重要作用。

背景

胰腺囊性病变包括炎性假囊肿、浆液性囊腺瘤和黏液性囊性肿瘤，其中一些是恶性的。先前使用免疫组化技术的研究表明，几乎所有胰腺黏液性肿瘤都表达pS2蛋白。pS2蛋白是一种生长因子，被认为在正常炎症和修复过程中起重要作用。我们测量了胰腺囊液中的pS2蛋白和其他生长因子，以评估它们潜在的病理生理和诊断意义。

方法

通过放射免疫测定法测量54份胰腺囊液中pS2蛋白的水平。使用商业免疫测定法测量22份囊液中的生长因子，即表皮生长因子（EGF）、转化生长因子-α（TGF-α）以及胰岛素样生长因子I和II（IGF-I、IGF-II）。

结果

黏液性囊肿囊液中pS2蛋白水平显著高于非黏液性病变，包括假囊肿和浆液性囊腺瘤（中位数：78,303 pg/mL；范围：218 - 361,176 pg/mL，相比之下，中位数：886 pg/mL；范围：0 - 14,206 pg/mL；p = 0.0001）。黏液性肿瘤中pS2的水平显著高于血浆值（中位数：392 pg/mL）。恶性黏液性病变中pS2蛋白水平往往高于良性黏液性囊肿，但这种差异无统计学意义（中位数：88,817对64,350 pg/mL；p = 0.159）。包括EGF、TGF-α、IGF-I和IGF-II在内的其他生长因子水平在不同类型囊肿之间无差异，且其值在正常血浆范围内。