Lee B N, Duvic M, Tang C K, Bueso-Ramos C, Estrov Z, Reuben J M
Division of Pathology and Laboratory Medicine, Division of Medicine, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
Clin Diagn Lab Immunol. 1999 Jan;6(1):79-84. doi: 10.1128/CDLI.6.1.79-84.1999.
Mycosis fungoides (MF) and Sezary syndrome (SS) are the two main clinical entities of cutaneous T-cell lymphoma (CTCL). As the disease progresses from MF to SS, a switch from a type 1 (interleukin [IL]-2 and gamma interferon [IFN-gamma]) to a type 2 (IL-4) cytokine production profile occurs. Although roles for type 1 and type 2 cytokines in the pathogenesis of CTCL have been proposed, the cellular origins of these cytokines are unclear. Using flow cytometry to identify individual T-cell subsets, we studied cytokine synthesis by the T cells of 13 patients with SS and 12 with MF and 9 hematologically healthy donors. Upon activation with phorbol 12-myristate 13-acetate (PMA), the numbers of T cells synthesizing IL-2 were similar for all study groups. Whereas the predominant T-cell producing IL-2 in healthy donors and in those with MF was CD7(+), in patients with SS, it was CD7(-). Although the number of IL-4(+) CD4(+) T cells was low for all study groups, there was a significantly higher number of IL-4(+) CD8(+) T cells in patients with MF than in those with SS or healthy donors. There was a decline in the number of IFN-gamma-producing T cells in CTCL donors compared to that in healthy donors. More importantly, there was a significant decrease in the number of IFN-gamma-producing T cells with disease progression from MF to SS. The inability of these T cells to synthesize IFN-gamma may have prognostic value in CTCL, since it may be responsible for the progression of the disease from MF to SS.
蕈样肉芽肿（MF）和塞扎里综合征（SS）是皮肤T细胞淋巴瘤（CTCL）的两个主要临床类型。随着疾病从MF进展到SS，细胞因子产生谱会从1型（白细胞介素[IL]-2和γ干扰素[IFN-γ]）转变为2型（IL-4）。尽管已经提出1型和2型细胞因子在CTCL发病机制中的作用，但这些细胞因子的细胞来源尚不清楚。我们使用流式细胞术鉴定单个T细胞亚群，研究了13例SS患者、12例MF患者和9名血液学健康供者的T细胞合成细胞因子的情况。在用佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）激活后，所有研究组中合成IL-2的T细胞数量相似。在健康供者和MF患者中，产生IL-2的主要T细胞是CD7(+)，而在SS患者中是CD7(-)。尽管所有研究组中IL-4(+) CD4(+) T细胞数量都很低，但MF患者中IL-4(+) CD8(+) T细胞数量显著高于SS患者或健康供者。与健康供者相比，CTCL供者中产生IFN-γ的T细胞数量有所下降。更重要的是，随着疾病从MF进展到SS，产生IFN-γ的T细胞数量显著减少。这些T细胞无法合成IFN-γ可能对CTCL具有预后价值，因为它可能是疾病从MF进展到SS的原因。
