良性和恶性淋巴增殖性皮肤病患者中具有CD4+CD7-免疫表型的循环T细胞的检测

Detection of circulating T cells with CD4+CD7- immunophenotype in patients with benign and malignant lymphoproliferative dermatoses.

作者信息

Harmon C B, Witzig T E, Katzmann J A, Pittelkow M R

机构信息

Department of Dermatology, Mayo Clinic, Rochester, MN 55905, USA.

出版信息

J Am Acad Dermatol. 1996 Sep;35(3 Pt 1):404-10. doi: 10.1016/s0190-9622(96)90605-2.

DOI:10.1016/s0190-9622(96)90605-2

PMID:8784277

Abstract

BACKGROUND

Cutaneous T-cell lymphomas (CTCLs) are malignancies of CD4+ T cells that involve the skin. CD4+CD7- cells may represent a malignant population in CTCL.

OBJECTIVE

Our purpose was to compare the percentage of CD4+CD7- cells and the expression of pan-T-cell antigens in blood lymphocytes from 31 patients with benign dermatoses with 35 patients who had CTCL.

METHODS

The patients with CTCL were classified as follows: 10 with mycosis fungoides (MF), seven with pre-Sézary syndrome (pre-SS), and 18 with Sézary syndrome (SS). Flow cytometry was used to determine the percentage of CD4+CD7- cells and the CD4/CD8 ratio and to detect aberrant expression of the pan-T-cell antigens CD2, CD3, and CD5.

RESULTS

We found a mean of 5.8% CD4+CD7- cells for the 16 normal control subjects and 9.3% for the benign cases (p = 0.13). The patients with pre-SS and SS had a higher percentage of CD4+CD7- cells (22.4% and 35.5%, respectively) than patients with benign dermatoses (p < 0.01); no difference was found between patients with benign dermatoses and those with MF (p = 0.80). The mean CD4/CD8 ratio was 3.1 for the normal control subjects compared with 7.4 for the patients with benign dermatoses (p < 0.01). Patients with SS had a ratio of 49, which was higher than the ratio for those with benign dermatoses (p < 0.01); however, the ratio for patients with MF and pre-SS did not differ from that of the group with benign dermatoses (p = 0.71 and 0.55, respectively). Aberrant CD2, CD3, or CD5 expression was observed in 66% of patients with SS, 29% with pre-SS, 30% with MF, but in none of the patients with a benign dermatosis.

CONCLUSION

Small numbers of CD4+CD7- cells can be found by flow cytometry in patients with a benign dermatosis and in normal control subjects. This T-cell subset is expanded in pre-SS and SS but not in MF. Aberrant pan-T-cell antigen expression is commonly observed in patients with SS but not in patients with a benign dermatosis.

摘要

背景

皮肤T细胞淋巴瘤（CTCL）是累及皮肤的CD4 + T细胞恶性肿瘤。CD4 + CD7-细胞可能代表CTCL中的恶性细胞群。

目的

我们的目的是比较31例良性皮肤病患者与35例CTCL患者血液淋巴细胞中CD4 + CD7-细胞的百分比以及泛T细胞抗原的表达。

方法

CTCL患者分类如下：10例蕈样肉芽肿（MF），7例Sezary综合征前期（pre-SS），18例Sezary综合征（SS）。采用流式细胞术测定CD4 + CD7-细胞百分比和CD4/CD8比值，并检测泛T细胞抗原CD2、CD3和CD5的异常表达。

结果

我们发现16名正常对照受试者的CD4 + CD7-细胞平均百分比为5.8%，良性病例为9.3%（p = 0.13）。pre-SS和SS患者的CD4 + CD7-细胞百分比（分别为22.4%和35.5%）高于良性皮肤病患者（p < 0.01）；良性皮肤病患者与MF患者之间未发现差异（p = 0.80）。正常对照受试者的平均CD4/CD8比值为3.1，而良性皮肤病患者为7.4（p < 0.01）。SS患者的比值为49，高于良性皮肤病患者（p < 0.01）；然而，MF和pre-SS患者的比值与良性皮肤病组无差异（分别为p = 0.71和0.55）。66%的SS患者、29%的pre-SS患者、30%的MF患者观察到CD2、CD3或CD5异常表达，但良性皮肤病患者均未观察到。