Lessin S R, Benoit B M, Li G, Moskovitz A, Zweiman B
Philadelphia Veterans Affairs Medical Center, Division of Allergy and Immunology, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA.
Clin Diagn Lab Immunol. 1999 Jan;6(1):85-8. doi: 10.1128/CDLI.6.1.85-88.1999.
To determine if functionally distinct T-lymphocyte (T cell) subsets accumulate in late-phase immunoglobulin E-mediated reactions (LPR), we quantitatively analyzed the immunophenotype and the T-cell receptor beta variable-gene (Vbeta) repertoire of T cells in cutaneous LPR. Peripheral blood and skin biopsies were obtained 6 or 24 h after sensitive subjects were challenged with intradermal injections of grass pollen allergen (Ag) and control (C) solution. The frequency of cells expressing CD3, CD4, CD8, CD45RO, and CD25/mm2 was determined by immunohistochemistry in nine subjects. Vbeta usage was assessed by reverse transcription-PCR in five of nine subjects. A significantly greater frequency of CD3(+) and CD45RO+ (memory) T cells was detected in Ag sites than in C sites at 24 h after challenge but not at 6 h. The frequency of activated (CD25(+)) and helper (CD4(+)) T cells appeared to be increased in Ag sites as well, though not significantly. Vbeta6 was the most commonly expressed Vbeta detected in Ag sites, but it was also detected in accompanying C sites. Vbeta2 was the most commonly expressed Vbeta detected in C sites. Sequence analysis in one case revealed Vbeta expression in a 6-h Ag site to be essentially polyclonal. Our findings suggest that memory T cells with Vbeta expression similar to that in normal skin accumulate in developing cutaneous LPR. The limited usage of Vbeta suggests a preferential recruitment or retention of reactive T cells from an endogenous subset of skin-homing T cells with its own skewed Vbeta repertoire.
为了确定功能不同的T淋巴细胞（T细胞）亚群是否在晚期免疫球蛋白E介导的反应（LPR）中积聚，我们定量分析了皮肤LPR中T细胞的免疫表型和T细胞受体β可变基因（Vβ）库。在敏感受试者皮内注射草花粉过敏原（Ag）和对照（C）溶液后6或24小时获取外周血和皮肤活检样本。通过免疫组织化学测定9名受试者中每平方毫米表达CD3、CD4、CD8、CD45RO和CD25的细胞频率。通过逆转录聚合酶链反应对9名受试者中的5名评估Vβ使用情况。激发后24小时，在Ag部位检测到的CD3(+)和CD45RO+（记忆）T细胞频率显著高于C部位，但6小时时未出现此情况。激活的（CD25(+)）和辅助性（CD4(+)）T细胞频率在Ag部位似乎也有所增加，尽管不显著。Vβ6是在Ag部位检测到的最常见表达的Vβ，但在相应的C部位也有检测到。Vβ2是在C部位检测到的最常见表达的Vβ。一例的序列分析显示，6小时Ag部位的Vβ表达基本为多克隆。我们的研究结果表明，在发展中的皮肤LPR中积聚了与正常皮肤中Vβ表达相似的记忆T细胞。Vβ的有限使用表明，从具有自身偏斜Vβ库的皮肤归巢T细胞内源性亚群中优先招募或保留了反应性T细胞。
