Bedard P M, Atkins P C, Zweiman B
J Allergy Clin Immunol. 1983 Apr;71(4):394-8. doi: 10.1016/0091-6749(83)90068-4.
We report comparisons of histamine release and neutrophil exudation in skin-window sites of 27 pollen-sensitive subjects challenged with antigen or buffer. More histamine was released within 30 min into appended collection chambers in antigen sites vs control sites. There were also more neutrophils adhering to membrane filters applied for 1 hr to the blister bases in antigen-challenged sites vs buffer sites. Comparison of skin-test extinction dilution titer, histamine release, and neutrophil accumulation in antigen-challenged sites in individual subjects showed that (1) there was no correlation between degrees of local histamine and neutrophil accumulation, (2) the increase in histamine but not in neutrophils correlated inversely with the concentration of antigen required to elicit a minimum wheal, and (3) both histamine and neutrophil increases were induced by antigen in a dose-dependent manner.
我们报告了27名花粉敏感受试者在接受抗原或缓冲液刺激后，皮肤窗口部位组胺释放和中性粒细胞渗出的比较情况。与对照部位相比，抗原部位在30分钟内有更多组胺释放到附加的收集室中。在抗原刺激部位与缓冲液部位相比，应用于水疱底部1小时的膜滤器上也有更多中性粒细胞黏附。对个体受试者抗原刺激部位的皮肤试验消退稀释滴度、组胺释放和中性粒细胞聚集进行比较，结果显示：（1）局部组胺和中性粒细胞聚集程度之间无相关性；（2）组胺增加而非中性粒细胞增加与引发最小风团所需抗原浓度呈负相关；（3）组胺和中性粒细胞增加均由抗原以剂量依赖性方式诱导。
