Werfel S, Massey W, Lichtenstein L M, Bochner B S
Department of Medicine, Johns Hopkins University School of Medicine, John Hopkins Asthma and Allergy Center, Baltimore, MD 21224-6801, USA.
J Allergy Clin Immunol. 1995 Jul;96(1):57-65. doi: 10.1016/s0091-6749(95)70033-1.
To determine whether activated memory T lymphocytes (CD3+CD4+CD45RO+CD25+HLA-DR+) preferentially accumulate during the cutaneous IgE-dependent late-phase response, we challenged 10 atopic patients with allergen and monitored the cellular influx for 12 hours with a skin blister chamber model. Four patients with allergy were also challenged with irrelevant allergen in control experiments. Histamine release and the size of the cutaneous late-phase response were measured. Light microscopic analysis and phenotyping of recruited and blood lymphocytes were performed with immunofluorescence and flow cytometry. Antigen challenge induced significant histamine release, a macroscopic late-phase response, and significant cellular influx in the appropriately challenged patients with allergy but not in control subjects. In the control group, only limited phenotypic analyses could be performed, which demonstrated an equivalent percentage of CD3+CD4+ cells in the chamber fluids compared with blood. In contrast, a higher proportion of CD4+ T lymphocytes and a lower proportion of CD8+ T lymphocytes accumulated in chamber fluids during the late-phase response compared with that present in blood. The vast majority of the recruited T lymphocytes expressed a memory phenotype (CD45RO+) with enhanced percentages of CD25+ and HLA-DR+ cells. Also, these cells had increased levels of very late antigen-4 (CD49d/CD29) and reduced levels of L-selectin compared with the same cells in blood. These results demonstrate that allergen challenge of the skin in patients with allergy leads to the preferential accumulation of activated, memory T lymphocytes. The mechanism by which these cells are selectively recruited during cutaneous allergic inflammation remains to be determined.
为了确定活化的记忆性T淋巴细胞（CD3+CD4+CD45RO+CD25+HLA-DR+）是否在皮肤IgE依赖性迟发反应期间优先聚集，我们用变应原激发了10名特应性患者，并使用皮肤水疱腔室模型监测细胞流入情况12小时。在对照实验中，还对4名过敏患者用无关变应原进行了激发。测量了组胺释放和皮肤迟发反应的大小。通过免疫荧光和流式细胞术对募集的淋巴细胞和血液淋巴细胞进行了光镜分析和表型分析。抗原激发在适当激发的过敏患者中诱导了显著的组胺释放、宏观迟发反应和显著的细胞流入，但在对照受试者中未出现。在对照组中，只能进行有限的表型分析，结果显示腔室液中CD3+CD4+细胞的百分比与血液中的相当。相比之下，与血液中的情况相比，迟发反应期间腔室液中积累的CD4+T淋巴细胞比例更高，而CD8+T淋巴细胞比例更低。绝大多数募集的T淋巴细胞表达记忆表型（CD45RO+），CD25+和HLA-DR+细胞的百分比增加。此外，与血液中的相同细胞相比，这些细胞的极晚期抗原-4（CD49d/CD29）水平升高，L-选择素水平降低。这些结果表明，变应原激发过敏患者的皮肤会导致活化的记忆性T淋巴细胞优先聚集。这些细胞在皮肤过敏性炎症期间被选择性募集的机制仍有待确定。
