Buck J, Sinclair M L, Schapal L, Cann M J, Levin L R
Department of Pharmacology, Joan and Sanford I. Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):79-84. doi: 10.1073/pnas.96.1.79.
Mammals have nine differentially regulated isoforms of G protein-responsive transmembrane-spanning adenylyl cyclases. We now describe the existence of a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or Forskolin regulation. Northern analysis indicates the gene encoding soluble adenylyl cyclase (sAC) is preferentially expressed in testis. As purified from rat testis cytosol, the active form of sAC appears to be a fragment derived from the full-length protein, suggesting a proteolytic mechanism for sAC activation. The two presumptive catalytic domains of sAC are closely related to cyanobacterial adenylyl cyclases, providing an evolutionary link between bacterial and mammalian signaling molecules.
哺乳动物有九种受不同调控的G蛋白反应性跨膜腺苷酸环化酶亚型。我们现在描述了一类独特的哺乳动物腺苷酸环化酶的存在，它是可溶性的，对G蛋白或福斯高林的调控不敏感。Northern分析表明，编码可溶性腺苷酸环化酶（sAC）的基因在睾丸中优先表达。从大鼠睾丸胞质溶胶中纯化得到的sAC活性形式似乎是全长蛋白衍生的片段，提示了sAC激活的蛋白水解机制。sAC的两个推定催化结构域与蓝藻腺苷酸环化酶密切相关，为细菌和哺乳动物信号分子之间提供了进化联系。
