Croul S, Radzievsky A, Sverstiuk A, Murray M
Department of Pathology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19102-1192, USA.
Exp Neurol. 1998 Nov;154(1):66-79. doi: 10.1006/exnr.1998.6875.
Quantitative receptor binding autoradiography was used to study the NK1, NMDA, 5HT1a, and 5HT2 receptor binding densities in the adult rat lumbar spinal cord from 3 days to 20 weeks following a unilateral crush lesion of the sciatic nerve. NK1 binding density increased unilaterally in the superficial dorsal horn on the side of the sciatic crush to reach levels 60% above controls by 4 weeks following the lesion and returned to control values by 12 weeks. NMDA binding density increased bilaterally and equally in both the dorsal and ventral horns to reach 300% of control values at 2 weeks following the crush and returned to near control values by 20 weeks following the lesion. Serotonergic receptor binding did not change. The changes in NK1 receptor binding density on postsynaptic dorsal horn cells are consistent with a response to the decrease and recovery in the synthesis and transport of tachykinins by the dorsal root ganglion cells following peripheral nerve injury. the bilateral changes in NMDA receptor binding are more likely mediated by polysynaptic pathways in the spinal cord that respond to the changes in metabolic events of the dorsal root ganglion cells evoked by axotomy and regeneration.
采用定量受体结合放射自显影术，研究成年大鼠坐骨神经单侧挤压损伤后3天至20周，其腰段脊髓中NK1、N-甲基-D-天冬氨酸（NMDA）、5-羟色胺1a（5HT1a）和5-羟色胺2（5HT2）受体的结合密度。坐骨神经挤压侧的浅背角NK1结合密度单侧增加，损伤后4周达到比对照组高60%的水平，损伤后12周恢复到对照值。挤压后2周，背角和腹角的NMDA结合密度双侧均同等增加，达到对照值的300%，损伤后20周恢复到接近对照值。5-羟色胺能受体结合未发生变化。突触后背角细胞上NK1受体结合密度的变化，与背根神经节细胞在周围神经损伤后速激肽合成和转运的减少及恢复的反应一致。NMDA受体结合的双侧变化更可能由脊髓中的多突触通路介导，该通路对轴突切断和再生引起的背根神经节细胞代谢事件的变化作出反应。
