Ryu B K, Ahn B O, Oh T Y, Kim S H, Kim W B, Lee E B
Research Laboratories, Dong-A Pharm. Co. Ltd., Yonginshi, Kyunggido, Korea.
Arch Pharm Res. 1998 Oct;21(5):508-13. doi: 10.1007/BF02975366.
The hepatoprotective effect of DA-9601, a quality-controlled extract of Artemisia asiatica, on liver damage induced by acetaminophen (APAP) and carbon tetrachloride (CCl4) was investigated by means of serum-biochemical, hepatic-biochemical, and histopathological examinations. Doses of DA-9601 (10, 30, or 100 mg/kg) were administered intragastrically to each rat on three consecutive days i.e. 48 h, 24 h and 2 h before a single administration of APAP (640 mg/kg, i.p.) or CCl4 (2 ml/kg, p.o.). Four h and 24 h after hepatotoxin treatment, the animals were sacrificed for evaluation of liver damage. Pretreatment of DA-9601 reduced the elevation of serum ALT, AST, LDH and histopathological changes such as centrilobular necrosis, vacuolar degeneration and inflammatory cell infiltration dose-dependently. DA-9601 also prevented APAP- and CCl4-induced hepatic glutathione (GSH) depletion and CCl4-induced increase of hepatic malondialdehyde (MDA), a parameter of lipid peroxidation, in a dose-dependent manner. These findings suggest that pretreatment with DA-9601 may reduce chemically induced liver injury by complex mechanisms which involve prevention of lipid peroxidation and preservation of hepatic GSH.
通过血清生化、肝脏生化和组织病理学检查，研究了质量控制的亚洲蒿提取物DA - 9601对乙酰氨基酚（APAP）和四氯化碳（CCl4）诱导的肝损伤的保肝作用。连续三天（即单次给予APAP（640 mg/kg，腹腔注射）或CCl4（2 ml/kg，口服）前48小时、24小时和2小时），给每只大鼠灌胃给予不同剂量（10、30或100 mg/kg）的DA - 9601。肝毒素处理后4小时和24小时，处死动物以评估肝损伤情况。DA - 9601预处理可剂量依赖性地降低血清ALT、AST、LDH的升高以及组织病理学变化，如小叶中心坏死、空泡变性和炎性细胞浸润。DA - 9601还可剂量依赖性地预防APAP和CCl4诱导的肝脏谷胱甘肽（GSH）消耗以及CCl4诱导的肝脏丙二醛（MDA，脂质过氧化参数）增加。这些发现表明，DA - 9601预处理可能通过涉及预防脂质过氧化和保存肝脏GSH的复杂机制减轻化学诱导的肝损伤。
