Loesch A, Milner P, Burnstock G
Department of Anatomy and Developmental Biology and Centre for Neuroscience, University College London, UK.
Neuroreport. 1998 Dec 1;9(17):3903-6. doi: 10.1097/00001756-199812010-00025.
The endothelium is recognized as a major source of endothelin in the vasculature. In this report we show for the first time that endothelin can be demonstrated with immunoelectronmicroscopy in perivascular nerves. About 36% of the axon profiles (varicosity/intervaricosity) examined in the rat basilar artery showed immunolabelling with a polyclonal antibody to endothelin-1. Endothelin-labelled axon varicosities were characterized by the presence of small spherical agranular vesicles (42 +/- 1 nm); some varicosities also contained large granular vesicles (92 +/- 5 nm) with labelled cores. Immunolabelling was mostly distributed in the axoplasm and in association with the membrane of synaptic vesicles (the lumen of the small agranular vesicles was immuno-negative). The presence of endothelin in perivascular nerves of the basilar artery suggests a neuronal as well as endothelial role in the physiological control of the vessel wall.
内皮细胞被认为是脉管系统中内皮素的主要来源。在本报告中，我们首次表明，采用免疫电子显微镜技术可在血管周围神经中证实内皮素的存在。在大鼠基底动脉中检测的轴突断面（膨体/节间）中，约36%显示出用抗内皮素-1多克隆抗体进行免疫标记。内皮素标记的轴突膨体的特征是存在小的球形无颗粒小泡（42±1nm）；一些膨体还含有大的有颗粒小泡（92±5nm），其核心有标记。免疫标记大多分布在轴浆中，并与突触小泡膜相关（小无颗粒小泡的腔免疫阴性）。基底动脉血管周围神经中内皮素的存在表明，在血管壁的生理控制中，神经元以及内皮细胞都发挥作用。
