兔C反应蛋白与含有暴露磷酸胆碱基团的支持脂质单层的钙依赖性结合。
Calcium-dependent binding of rabbit C-reactive protein to supported lipid monolayers containing exposed phosphorylcholine group.
作者信息
Sui S F, Sun Y T, Mi L Z
机构信息
Biophysics Group, State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of
出版信息
Biophys J. 1999 Jan;76(1 Pt 1):333-41. doi: 10.1016/S0006-3495(99)77200-0.
The interaction of rabbit C-reactive protein (rCRP) with a supported monolayer containing a phosphorylcholine moiety was studied. Three types of phospholipids were synthesized, each containing a insertion spacer of eight, six, or three atoms between the phosphorylcholine group and hydrophobic tail. By varying the length of the insertion spacer, we can vary the extension of the phosphorylcholine group from the membrane surface. By varying the monolayer composition, we can control the lateral distance between the exposed phosphorylcholine groups. Using the surface plasmon resonance technique (SPR), we demonstrated that the calcium-dependent binding of rCRP to the model membrane is governed not only by the ability of the ligand to access the binding pocket fully (spacer length), but also by lateral hindrance within the two-dimensional plane of the membrane. The value of the apparent binding constant was estimated by theoretical analysis, which is obviously dependent on the composition of the lipid mixture, and a maximum of (9.9 +/- 1.5) x 10(6) M-1 was obtained.
研究了兔C反应蛋白（rCRP）与含有磷酰胆碱部分的支撑单层之间的相互作用。合成了三种类型的磷脂，每种磷脂在磷酰胆碱基团与疏水尾部之间含有八个、六个或三个原子的插入间隔基团。通过改变插入间隔基团的长度，可以改变磷酰胆碱基团从膜表面的伸展程度。通过改变单层的组成，可以控制暴露的磷酰胆碱基团之间的侧向距离。使用表面等离子体共振技术（SPR），我们证明了rCRP与模型膜的钙依赖性结合不仅受配体完全进入结合口袋的能力（间隔基团长度）的控制，还受膜二维平面内的侧向阻碍的控制。通过理论分析估计了表观结合常数的值，该值明显取决于脂质混合物的组成，并且获得的最大值为(9.9±1.5)×10⁶ M⁻¹。
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